Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1688895..1689522 Replicon chromosome
Accession NZ_CP050865
Organism Escherichia coli strain 8-3-Ti3

Toxin (Protein)


Gene name higB Uniprot ID A0A828BKW8
Locus tag HFC66_RS08540 Protein ID WP_024258354.1
Coordinates 1688895..1689224 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID Q32DW6
Locus tag HFC66_RS08545 Protein ID WP_000147081.1
Coordinates 1689214..1689522 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HFC66_RS08480 1683939..1684406 - 468 WP_167839448.1 lysis protein -
HFC66_RS08485 1684403..1684900 - 498 WP_032359927.1 lysozyme RrrD -
HFC66_RS08490 1684900..1685115 - 216 WP_000839570.1 class II holin family protein -
HFC66_RS08505 1685609..1686232 - 624 WP_001235461.1 antitermination protein -
HFC66_RS08510 1686229..1686417 - 189 WP_000994516.1 protein ninH -
HFC66_RS08515 1686414..1686776 - 363 WP_073840903.1 RusA family crossover junction endodeoxyribonuclease -
HFC66_RS08520 1686773..1687543 - 771 WP_001344365.1 phage antirepressor Ant -
HFC66_RS08525 1687558..1687794 - 237 Protein_1663 Rha family transcriptional regulator -
HFC66_RS08530 1687869..1688573 - 705 WP_073840904.1 ORF6N domain-containing protein -
HFC66_RS08535 1688624..1688849 - 226 Protein_1665 ash family protein -
HFC66_RS08540 1688895..1689224 + 330 WP_024258354.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HFC66_RS08545 1689214..1689522 + 309 WP_000147081.1 helix-turn-helix transcriptional regulator Antitoxin
HFC66_RS08550 1689564..1689740 - 177 WP_001254220.1 NinE family protein -
HFC66_RS08555 1689737..1690177 - 441 WP_000736913.1 recombination protein NinB -
HFC66_RS08560 1690255..1693161 - 2907 WP_073840460.1 toprim domain-containing protein -
HFC66_RS08565 1693392..1693829 - 438 Protein_1671 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - ipaH4.5 1675226..1710997 35771


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-97)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12837.87 Da        Isoelectric Point: 9.9966

>T153411 WP_024258354.1 NZ_CP050865:1688895-1689224 [Escherichia coli]
MNYTIEYYSEDVRLEVDRLPLSMRARYQHLVERMKIYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIML
HSFVKKTQKTPPAERKKAETRMKEVKHDW

Download         Length: 330 bp

>T153411 NZ_CP066900:c4777327-4776794 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11185.94 Da        Isoelectric Point: 8.5840

>AT153411 WP_000147081.1 NZ_CP050865:1689214-1689522 [Escherichia coli]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Download         Length: 309 bp

>AT153411 NZ_CP066900:c4777604-4777338 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828BKW8

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TJC1

References