Detailed information of TA system TA153410

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 1522360..1522901
• Replicon: chromosome
• Accession: NZ_CP050865
• Organism: Escherichia coli strain 8-3-Ti3

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: S1FHQ9
• Locus tag: HFC66_RS07715
• Protein ID: WP_000615979.1
• Coordinates: 1522623..1522901 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: HFC66_RS07710
• Protein ID: WP_000729707.1
• Coordinates: 1522360..1522620 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HFC66_RS07685	1517396..1518535	+	1140	WP_000785662.1	flagellar type III secretion system protein FlhB	-
HFC66_RS07690	1518519..1520612	+	2094	WP_073849380.1	flagellar biosynthesis protein FlhA	-
HFC66_RS07695	1520717..1520995	+	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
HFC66_RS07700	1520988..1521344	+	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-
HFC66_RS07705	1521401..1522174	-	774	WP_000543899.1	C40 family peptidase	-
HFC66_RS07710	1522360..1522620	+	261	WP_000729707.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
HFC66_RS07715	1522623..1522901	+	279	WP_000615979.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
HFC66_RS07720	1523057..1523797	+	741	WP_001225679.1	murein L,D-transpeptidase	-
HFC66_RS07725	1523768..1524535	-	768	WP_001365365.1	class II glutamine amidotransferase	-
HFC66_RS07730	1524741..1525319	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10830.62 Da
• Isoelectric Point: 10.0702

>T153410 WP_000615979.1 NZ_CP050865:1522623-1522901 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T153410 NZ_CP066900:4164495-4164713 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9421.80 Da
• Isoelectric Point: 5.0758

>AT153410 WP_000729707.1 NZ_CP050865:1522360-1522620 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKTKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT153410 NZ_CP066900:4164094-4164468 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: S1FHQ9