Detailed information of TA system TA153403

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4613105..4613707
• Replicon: chromosome
• Accession: NZ_CP050862
• Organism: Escherichia coli strain 8-3-DC15

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HFC67_RS23545
• Protein ID: WP_000897305.1
• Coordinates: 4613105..4613416 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HFC67_RS23550
• Protein ID: WP_000356397.1
• Coordinates: 4613417..4613707 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HFC67_RS23520	4609356..4610177	+	822	Protein_4583	hypothetical protein	-
HFC67_RS23525	4610063..4610335	-	273	WP_167839330.1	hypothetical protein	-
HFC67_RS23530	4610234..4610931	+	698	Protein_4585	IS1 family transposase	-
HFC67_RS23535	4610952..4611800	+	849	WP_167839485.1	transposase	-
HFC67_RS23540	4611968..4612876	-	909	WP_032360143.1	alpha/beta hydrolase	-
HFC67_RS23545	4613105..4613416	+	312	WP_000897305.1	hypothetical protein	Toxin
HFC67_RS23550	4613417..4613707	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HFC67_RS23555	4614293..4614511	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
HFC67_RS23560	4614662..4615359	+	698	Protein_4591	IS1 family transposase	-
HFC67_RS23565	4615507..4615749	+	243	WP_001086388.1	hypothetical protein	-
HFC67_RS23570	4616079..4617008	-	930	WP_032359289.1	formate dehydrogenase accessory protein FdhE	-
HFC67_RS23575	4617005..4617640	-	636	WP_073840738.1	formate dehydrogenase cytochrome b556 subunit	-
HFC67_RS23580	4617637..4618539	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	4610428..4615359	4931

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T153403 WP_000897305.1 NZ_CP050862:4613105-4613416 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T153403 NZ_CP066899:2792-3058 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT153403 WP_000356397.1 NZ_CP050862:4613417-4613707 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT153403 NZ_CP066899:2584-2808 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7