Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3816919..3817718 Replicon chromosome
Accession NZ_CP050862
Organism Escherichia coli strain 8-3-DC15

Toxin (Protein)


Gene name yhaV Uniprot ID A0A3I9P420
Locus tag HFC67_RS19550 Protein ID WP_000347271.1
Coordinates 3817254..3817718 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID C3ST72
Locus tag HFC67_RS19545 Protein ID WP_001302819.1
Coordinates 3816919..3817254 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HFC67_RS19530 3812704..3813474 - 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -
HFC67_RS19535 3813490..3814824 - 1335 WP_167839460.1 galactarate/glucarate/glycerate transporter GarP -
HFC67_RS19540 3815199..3816770 + 1572 WP_001273770.1 galactarate dehydratase -
HFC67_RS19545 3816919..3817254 + 336 WP_001302819.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HFC67_RS19550 3817254..3817718 + 465 WP_000347271.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HFC67_RS19555 3817773..3818582 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HFC67_RS19560 3818831..3820111 + 1281 WP_032359524.1 tagatose-bisphosphate aldolase subunit KbaZ -
HFC67_RS19565 3820134..3820607 + 474 WP_016245132.1 PTS N-acetylgalactosamine transporter subunit IIB -
HFC67_RS19570 3820618..3821397 + 780 WP_000406215.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HFC67_RS19575 3821387..3822265 + 879 WP_001380079.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HFC67_RS19580 3822283..3822717 + 435 WP_000948814.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3807328..3817718 10390


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17849.25 Da        Isoelectric Point: 9.6924

>T153401 WP_000347271.1 NZ_CP050862:3817254-3817718 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEENH

Download         Length: 465 bp

>T153401 NZ_CP066897:126487-126636 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12388.97 Da        Isoelectric Point: 4.8616

>AT153401 WP_001302819.1 NZ_CP050862:3816919-3817254 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT153401 NZ_CP066897:c126442-126383 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3I9P420

Antitoxin

Source ID Structure

References