Detailed information of TA system TA153400

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3441273..3441900
• Replicon: chromosome
• Accession: NZ_CP050862
• Organism: Escherichia coli strain 8-3-DC15

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A828BKW8
• Locus tag: HFC67_RS17685
• Protein ID: WP_024258354.1
• Coordinates: 3441273..3441602 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q32DW6
• Locus tag: HFC67_RS17690
• Protein ID: WP_000147081.1
• Coordinates: 3441592..3441900 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HFC67_RS17625	3436317..3436784	-	468	WP_167839448.1	lysis protein	-
HFC67_RS17630	3436781..3437278	-	498	WP_032359927.1	lysozyme RrrD	-
HFC67_RS17635	3437278..3437493	-	216	WP_000839570.1	class II holin family protein	-
HFC67_RS17650	3437987..3438610	-	624	WP_001235461.1	antitermination protein	-
HFC67_RS17655	3438607..3438795	-	189	WP_000994516.1	protein ninH	-
HFC67_RS17660	3438792..3439154	-	363	WP_073840903.1	RusA family crossover junction endodeoxyribonuclease	-
HFC67_RS17665	3439151..3439921	-	771	WP_001344365.1	phage antirepressor Ant	-
HFC67_RS17670	3439936..3440172	-	237	Protein_3447	Rha family transcriptional regulator	-
HFC67_RS17675	3440247..3440951	-	705	WP_073840904.1	ORF6N domain-containing protein	-
HFC67_RS17680	3441002..3441227	-	226	Protein_3449	ash family protein	-
HFC67_RS17685	3441273..3441602	+	330	WP_024258354.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HFC67_RS17690	3441592..3441900	+	309	WP_000147081.1	helix-turn-helix transcriptional regulator	Antitoxin
HFC67_RS17695	3441942..3442118	-	177	WP_001254220.1	NinE family protein	-
HFC67_RS17700	3442115..3442555	-	441	WP_000736913.1	recombination protein NinB	-
HFC67_RS17705	3442633..3445539	-	2907	WP_073840460.1	toprim domain-containing protein	-
HFC67_RS17710	3445770..3446207	-	438	Protein_3455	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ipaH4.5	3427604..3463375	35771

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12837.87 Da
• Isoelectric Point: 9.9966

>T153400 WP_024258354.1 NZ_CP050862:3441273-3441602 [Escherichia coli]
MNYTIEYYSEDVRLEVDRLPLSMRARYQHLVERMKIYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIML
HSFVKKTQKTPPAERKKAETRMKEVKHDW

Nucleotide

• Length: 330 bp

>T153400 NZ_CP066897:c51978-51562 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11185.94 Da
• Isoelectric Point: 8.5840

>AT153400 WP_000147081.1 NZ_CP050862:3441592-3441900 [Escherichia coli]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Nucleotide

• Length: 309 bp

>AT153400 NZ_CP066897:c52205-51975 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A828BKW8

Antitoxin

• AlphaFold DB: A0A4P7TJC1