Detailed information of TA system TA153399

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 3274738..3275279
• Replicon: chromosome
• Accession: NZ_CP050862
• Organism: Escherichia coli strain 8-3-DC15

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: S1FHQ9
• Locus tag: HFC67_RS16860
• Protein ID: WP_000615979.1
• Coordinates: 3275001..3275279 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: HFC67_RS16855
• Protein ID: WP_000729707.1
• Coordinates: 3274738..3274998 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HFC67_RS16830	3269774..3270913	+	1140	WP_000785662.1	flagellar type III secretion system protein FlhB	-
HFC67_RS16835	3270897..3272990	+	2094	WP_073849380.1	flagellar biosynthesis protein FlhA	-
HFC67_RS16840	3273095..3273373	+	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
HFC67_RS16845	3273366..3273722	+	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-
HFC67_RS16850	3273779..3274552	-	774	WP_000543899.1	C40 family peptidase	-
HFC67_RS16855	3274738..3274998	+	261	WP_000729707.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
HFC67_RS16860	3275001..3275279	+	279	WP_000615979.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
HFC67_RS16865	3275435..3276175	+	741	WP_001225679.1	murein L,D-transpeptidase	-
HFC67_RS16870	3276146..3276913	-	768	WP_001365365.1	class II glutamine amidotransferase	-
HFC67_RS16875	3277119..3277697	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10830.62 Da
• Isoelectric Point: 10.0702

>T153399 WP_000615979.1 NZ_CP050862:3275001-3275279 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T153399 NZ_CP066896:196129-196611 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9421.80 Da
• Isoelectric Point: 5.0758

>AT153399 WP_000729707.1 NZ_CP050862:3274738-3274998 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKTKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT153399 NZ_CP066896:195875-196141 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: S1FHQ9