Detailed information of TA system TA153379

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4668013..4668610
• Replicon: chromosome
• Accession: NZ_CP050858
• Organism: Klebsiella pneumoniae strain SCH6109

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: HC233_RS23175
• Protein ID: WP_004893639.1
• Coordinates: 4668293..4668610 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: HC233_RS23170
• Protein ID: WP_004142561.1
• Coordinates: 4668013..4668300 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC233_RS23140	4664220..4664468	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
HC233_RS23145	4664486..4664827	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
HC233_RS23150	4664858..4665973	-	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
HC233_RS23155	4666153..4666734	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
HC233_RS23160	4666734..4667102	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
HC233_RS23165	4667222..4667875	+	654	WP_167835824.1	oxygen-insensitive NAD(P)H nitroreductase	-
HC233_RS23170	4668013..4668300	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
HC233_RS23175	4668293..4668610	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HC233_RS23180	4668795..4669838	-	1044	WP_023279204.1	DUF2157 domain-containing protein	-
HC233_RS23185	4670508..4671374	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
HC233_RS23190	4671484..4672911	+	1428	WP_105910454.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4668013..4677168	9155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T153379 WP_004893639.1 NZ_CP050858:c4668610-4668293 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T153379 NZ_CP066891:c4748483-4747950 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT153379 WP_004142561.1 NZ_CP050858:c4668300-4668013 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT153379 NZ_CP066891:c4748760-4748494 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1