Detailed information of TA system TA153367

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1100560..1101206
• Replicon: chromosome
• Accession: NZ_CP050858
• Organism: Klebsiella pneumoniae strain SCH6109

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HC233_RS05305
• Protein ID: WP_167835484.1
• Coordinates: 1100560..1100907 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A483GM64
• Locus tag: HC233_RS05310
• Protein ID: WP_004188315.1
• Coordinates: 1100907..1101206 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC233_RS05295	1096485..1097918	+	1434	WP_002920564.1	glycogen synthase GlgA	-
HC233_RS05300	1097936..1100384	+	2449	Protein_998	glycogen phosphorylase	-
HC233_RS05305	1100560..1100907	+	348	WP_167835484.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HC233_RS05310	1100907..1101206	+	300	WP_004188315.1	XRE family transcriptional regulator	Antitoxin
HC233_RS05315	1101269..1102777	-	1509	WP_002920554.1	glycerol-3-phosphate dehydrogenase	-
HC233_RS05320	1102982..1103311	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
HC233_RS05325	1103362..1104192	+	831	WP_004188317.1	rhomboid family intramembrane serine protease GlpG	-
HC233_RS05330	1104242..1105000	+	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-78)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13449.44 Da
• Isoelectric Point: 6.2327

>T153367 WP_167835484.1 NZ_CP050858:1100560-1100907 [Klebsiella pneumoniae]
MWDVETTDTFDTSFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T153367 NZ_CP066887:c5371630-5371247 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10873.64 Da
• Isoelectric Point: 10.1464

>AT153367 WP_004188315.1 NZ_CP050858:1100907-1101206 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAAWG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT153367 NZ_CP066887:c5371872-5371630 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A483GM64