Detailed information of TA system TA153330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2123547..2124274
• Replicon: chromosome
• Accession: NZ_CP050845
• Organism: Klebsiella pneumoniae strain Bckp206

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: HC704_RS10570
• Protein ID: WP_011251285.1
• Coordinates: 2123547..2123858 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HC704_RS10575
• Protein ID: WP_011251286.1
• Coordinates: 2123855..2124274 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC704_RS10540	2118777..2119757	+	981	WP_048293850.1	IS5-like element ISKpn26 family transposase	-
HC704_RS10545	2120303..2120482	-	180	Protein_2016	IS5/IS1182 family transposase	-
HC704_RS10550	2120522..2122060	-	1539	WP_032430751.1	IS66 family transposase	-
HC704_RS10555	2122109..2122456	-	348	WP_032430752.1	IS66 family insertion sequence element accessory protein TnpB	-
HC704_RS10560	2122453..2122791	-	339	WP_023288266.1	IS66 family insertion sequence hypothetical protein	-
HC704_RS10565	2122884..2123342	+	459	Protein_2020	DDE-type integrase/transposase/recombinase	-
HC704_RS10570	2123547..2123858	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
HC704_RS10575	2123855..2124274	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
HC704_RS10580	2124344..2124646	+	303	WP_167813119.1	hypothetical protein	-
HC704_RS10590	2124852..2125820	-	969	WP_074170039.1	IS5-like element IS903B family transposase	-
HC704_RS10595	2125852..2126187	-	336	Protein_2025	conjugal transfer protein TraI	-
HC704_RS10600	2126201..2126989	-	789	WP_101979139.1	hypothetical protein	-
HC704_RS10605	2126989..2127453	-	465	WP_048291880.1	hypothetical protein	-
HC704_RS10610	2128141..2129109	+	969	WP_004099053.1	IS5-like element IS903B family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	cheD	2114901..2131028	16127
	-	inside	IScluster/Tn	-	cheD	2114901..2129109	14208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T153330 WP_011251285.1 NZ_CP050845:2123547-2123858 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T153330 NZ_CP066845:49273-49671 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT153330 WP_011251286.1 NZ_CP050845:2123855-2124274 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT153330 NZ_CP066845:49046-49273 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1