Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 174609..175336 Replicon plasmid pBckp186
Accession NZ_CP050844
Organism Klebsiella pneumoniae strain Bckp186

Toxin (Protein)


Gene name higB Uniprot ID A0A7W3D9W1
Locus tag HC697_RS27415 Protein ID WP_011251285.1
Coordinates 174609..174920 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HC697_RS27420 Protein ID WP_011251286.1
Coordinates 174917..175336 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HC697_RS27390 171060..172010 + 951 WP_167829917.1 IS5-like element IS903B family transposase -
HC697_RS27395 172086..173122 - 1037 Protein_174 IS66 family transposase -
HC697_RS27400 173171..173518 - 348 WP_004114612.1 IS66 family insertion sequence element accessory protein TnpB -
HC697_RS27405 173515..173853 - 339 WP_135733211.1 transposase -
HC697_RS27410 173946..174404 + 459 Protein_177 DDE-type integrase/transposase/recombinase -
HC697_RS27415 174609..174920 + 312 WP_011251285.1 type II toxin-antitoxin system HigB family toxin Toxin
HC697_RS27420 174917..175336 + 420 WP_011251286.1 helix-turn-helix domain-containing protein Antitoxin
HC697_RS27425 175576..175901 - 326 Protein_180 transposase -
HC697_RS27430 175989..177011 + 1023 WP_094320218.1 IS21 family transposase -
HC697_RS27435 177008..177790 + 783 WP_032437422.1 IS21-like element ISSen3 family helper ATPase IstB -
HC697_RS27440 177857..178084 + 228 Protein_183 hypothetical protein -
HC697_RS27445 178521..180062 + 1542 Protein_184 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..223022 223022
- inside IScluster/Tn - - 157785..190783 32998


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12346.12 Da        Isoelectric Point: 9.7248

>T153319 WP_011251285.1 NZ_CP050844:174609-174920 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Download         Length: 312 bp

>T153319 NZ_CP066844:2658265-2658372 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15377.46 Da        Isoelectric Point: 4.4420

>AT153319 WP_011251286.1 NZ_CP050844:174917-175336 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT153319 NZ_CP066844:c2658212-2658151 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3D9W1

Antitoxin

Source ID Structure

References