Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 350490..351136 Replicon chromosome
Accession NZ_CP050843
Organism Klebsiella pneumoniae strain Bckp186

Toxin (Protein)


Gene name higB Uniprot ID A0A332YAY3
Locus tag HC697_RS01635 Protein ID WP_032435150.1
Coordinates 350490..350837 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A231WWU8
Locus tag HC697_RS01640 Protein ID WP_004174017.1
Coordinates 350837..351136 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HC697_RS01625 346416..347849 + 1434 WP_002920564.1 glycogen synthase GlgA -
HC697_RS01630 347867..350314 + 2448 WP_002920561.1 glycogen phosphorylase -
HC697_RS01635 350490..350837 + 348 WP_032435150.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HC697_RS01640 350837..351136 + 300 WP_004174017.1 XRE family transcriptional regulator Antitoxin
HC697_RS01645 351199..352707 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
HC697_RS01650 352912..353241 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
HC697_RS01655 353292..354122 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
HC697_RS01660 354172..354930 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13531.52 Da        Isoelectric Point: 5.6749

>T153300 WP_032435150.1 NZ_CP050843:350490-350837 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATILILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T153300 NZ_CP066839:c3888611-3888213 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10844.64 Da        Isoelectric Point: 10.1464

>AT153300 WP_004174017.1 NZ_CP050843:350837-351136 [Klebsiella pneumoniae]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQTDNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT153300 NZ_CP066839:c3888907-3888614 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332YAY3

Antitoxin

Source ID Structure
AlphaFold DB A0A231WWU8

References