Detailed information of TA system TA153277

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1498..2225
• Replicon: plasmid pBckp021-2
• Accession: NZ_CP050836
• Organism: Klebsiella pneumoniae strain Bckp021

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: HC680_RS28005
• Protein ID: WP_011251285.1
• Coordinates: 1914..2225 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HC680_RS28000
• Protein ID: WP_011251286.1
• Coordinates: 1498..1917 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC680_RS27990	1..969	+	969	WP_074168518.1	IS5-like element IS903B family transposase	-
HC680_RS27995	1015..1428	-	414	WP_017901337.1	hypothetical protein	-
HC680_RS28000	1498..1917	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
HC680_RS28005	1914..2225	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
HC680_RS28010	2430..2888	-	459	Protein_4	DDE-type integrase/transposase/recombinase	-
HC680_RS28015	2981..3358	+	378	WP_004118218.1	IS66 family insertion sequence hypothetical protein	-
HC680_RS28020	3355..3702	+	348	WP_032430752.1	IS66 family insertion sequence element accessory protein TnpB	-
HC680_RS28025	3751..5289	+	1539	WP_017901237.1	IS66 family transposase	-
HC680_RS28030	5329..5508	+	180	Protein_8	IS5/IS1182 family transposase	-
HC680_RS28035	6054..7034	-	981	WP_000019441.1	IS5-like element ISKpn26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaSHV-12	-	1..237942	237942
	-	inside	IScluster/Tn	-	-	1..26315	26314

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T153277 WP_011251285.1 NZ_CP050836:c2225-1914 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T153277 NZ_CP066836:1883129-1883347 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT153277 WP_011251286.1 NZ_CP050836:c1917-1498 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT153277 NZ_CP066836:1882729-1883103 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1