Detailed information of TA system TA153254

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 158710..159437
• Replicon: plasmid pBckp067
• Accession: NZ_CP050830
• Organism: Klebsiella pneumoniae strain Bckp067

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: HC709_RS27240
• Protein ID: WP_011251285.1
• Coordinates: 159126..159437 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HC709_RS27235
• Protein ID: WP_011251286.1
• Coordinates: 158710..159129 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC709_RS27215	155962..156189	-	228	Protein_149	hypothetical protein	-
HC709_RS27220	156256..157038	-	783	WP_032437422.1	IS21-like element ISSen3 family helper ATPase IstB	-
HC709_RS27225	157035..158057	-	1023	WP_094320218.1	IS21 family transposase	-
HC709_RS27230	158145..158470	+	326	Protein_152	transposase	-
HC709_RS27235	158710..159129	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
HC709_RS27240	159126..159437	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
HC709_RS27245	159642..160100	-	459	Protein_155	DDE-type integrase/transposase/recombinase	-
HC709_RS27250	160193..160531	+	339	WP_135733211.1	transposase	-
HC709_RS27255	160528..160875	+	348	WP_004114612.1	IS66 family insertion sequence element accessory protein TnpB	-
HC709_RS27260	160924..161960	+	1037	Protein_158	IS66 family transposase	-
HC709_RS27265	162048..163073	+	1026	WP_001101446.1	IS110 family transposase	-
HC709_RS27270	163410..164360	-	951	WP_167829917.1	IS5-like element IS903B family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..222783	222783
	-	inside	IScluster/Tn	-	-	156256..177635	21379

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T153254 WP_011251285.1 NZ_CP050830:c159437-159126 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T153254 NZ_CP066815:4524-4811 [Aeromonas caviae]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT153254 WP_011251286.1 NZ_CP050830:c159129-158710 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT153254 NZ_CP066815:4798-5139 [Aeromonas caviae]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAATCATCAATAAGAATGG
GAGCTTAGGCTCCCATTTTTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1