Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3896611..3897208 Replicon chromosome
Accession NZ_CP050829
Organism Klebsiella pneumoniae strain Bckp067

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag HC709_RS19580 Protein ID WP_004142563.1
Coordinates 3896891..3897208 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag HC709_RS19575 Protein ID WP_004142561.1
Coordinates 3896611..3896898 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HC709_RS19545 3892691..3892939 + 249 WP_002893037.1 DUF1158 domain-containing protein -
HC709_RS19550 3892957..3893298 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
HC709_RS19555 3893329..3894444 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
HC709_RS19560 3894624..3895205 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
HC709_RS19565 3895205..3895573 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
HC709_RS19570 3895693..3896346 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
HC709_RS19575 3896611..3896898 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
HC709_RS19580 3896891..3897208 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HC709_RS19585 3897393..3898436 - 1044 WP_023284821.1 DUF2157 domain-containing protein -
HC709_RS19590 3899103..3899969 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
HC709_RS19595 3900078..3901505 + 1428 WP_102001330.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T153246 WP_004142563.1 NZ_CP050829:c3897208-3896891 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T153246 NZ_CP066809:3111022-3111252 [Shigella flexneri 3a]
ATGAAGGTATACGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGGCTTTTCGAACTCAGAATTAAAGG
CAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAACGCATCATCATGCTGCATAGTTTTGTAAAGAAAA
CGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCATGACTGGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT153246 WP_004142561.1 NZ_CP050829:c3896898-3896611 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT153246 NZ_CP066809:3111242-3111550 [Shigella flexneri 3a]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACACTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References