Detailed information of TA system TA153229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 14678..15405
• Replicon: plasmid pBckp212-1
• Accession: NZ_CP050827
• Organism: Klebsiella pneumoniae strain Bckp212

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: HC649_RS25945
• Protein ID: WP_011251285.1
• Coordinates: 15094..15405 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HC649_RS25940
• Protein ID: WP_011251286.1
• Coordinates: 14678..15097 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC649_RS25905	10537..10890	-	354	WP_017146595.1	hypothetical protein	-
HC649_RS25910	10883..11083	-	201	WP_024193481.1	hypothetical protein	-
HC649_RS25915	11623..12141	-	519	Protein_11	methyl-accepting chemotaxis protein II	-
HC649_RS25920	12212..13099	+	888	Protein_12	IS5 family transposase	-
HC649_RS25925	13132..14100	+	969	WP_167813116.1	IS5-like element IS903B family transposase	-
HC649_RS25935	14306..14608	-	303	WP_167813119.1	hypothetical protein	-
HC649_RS25940	14678..15097	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
HC649_RS25945	15094..15405	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
HC649_RS25950	15610..16068	-	459	Protein_17	DDE-type integrase/transposase/recombinase	-
HC649_RS25955	16161..16499	+	339	WP_023288266.1	IS66 family insertion sequence hypothetical protein	-
HC649_RS25960	16496..16843	+	348	WP_032430752.1	IS66 family insertion sequence element accessory protein TnpB	-
HC649_RS25965	16892..18430	+	1539	WP_032430751.1	IS66 family transposase	-
HC649_RS25970	18470..18649	+	180	Protein_21	IS5/IS1182 family transposase	-
HC649_RS25975	19195..20175	-	981	WP_048293850.1	IS5-like element ISKpn26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3'')-Ib / aph(6)-Id	cheD	1..186278	186278
	-	inside	IScluster/Tn	-	cheD	8291..20175	11884

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T153229 WP_011251285.1 NZ_CP050827:c15405-15094 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T153229 NZ_CP066806:c4361707-4361357 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGACTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT153229 WP_011251286.1 NZ_CP050827:c15097-14678 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT153229 NZ_CP066806:c4361952-4361701 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1