Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4575912..4576558 Replicon chromosome
Accession NZ_CP050826
Organism Klebsiella pneumoniae strain Bckp212

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag HC649_RS22855 Protein ID WP_117108859.1
Coordinates 4576208..4576558 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HC649_RS22850 Protein ID WP_117108858.1
Coordinates 4575912..4576211 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HC649_RS22825 4570968..4571429 - 462 WP_048337979.1 hypothetical protein -
HC649_RS22830 4571404..4572300 - 897 WP_048337978.1 hypothetical protein -
HC649_RS22835 4573009..4574280 - 1272 WP_048337977.1 integrase arm-type DNA-binding domain-containing protein -
HC649_RS22840 4574547..4575125 - 579 WP_117108857.1 hypothetical protein -
HC649_RS22845 4575294..4575619 + 326 Protein_4393 hypothetical protein -
HC649_RS22850 4575912..4576211 - 300 WP_117108858.1 helix-turn-helix transcriptional regulator Antitoxin
HC649_RS22855 4576208..4576558 - 351 WP_117108859.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HC649_RS22860 4576969..4578012 - 1044 WP_117108900.1 helicase RepA family protein -
HC649_RS22865 4578095..4578280 - 186 WP_117108860.1 AlpA family phage regulatory protein -
HC649_RS22870 4578305..4579573 - 1269 WP_117108861.1 tyrosine-type recombinase/integrase -
HC649_RS22875 4579791..4580693 - 903 WP_181958951.1 site-specific integrase -
HC649_RS22880 4580686..4580916 - 231 WP_117108901.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4544350..4600286 55936


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-80)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13704.66 Da        Isoelectric Point: 5.9108

>T153226 WP_117108859.1 NZ_CP050826:c4576558-4576208 [Klebsiella pneumoniae]
MWTVLFSQRFDDWLSEQDDALQEKVLADLGKLQMYDPELPRPYADTLKGSRYKNMKELRIQFSGQPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYEKLLRIAEDEFAAHLKNLEINQ

Download         Length: 351 bp

>T153226 NZ_CP066806:c4214604-4214317 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11313.22 Da        Isoelectric Point: 5.1767

>AT153226 WP_117108858.1 NZ_CP050826:c4576211-4575912 [Klebsiella pneumoniae]
MRTLDDVITARSPESQTRIKEMFDEMILEVGLQMMREELQLSQKYVAEMMGVSQPAITQLEQRGNDLKLATLKRYIEAMG
GKLSLDVELPTGKRIAFHI

Download         Length: 300 bp

>AT153226 NZ_CP066806:c4214330-4214028 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCCTCGGTGCGTATTGATTCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAATTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References