Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3748083..3748680 Replicon chromosome
Accession NZ_CP050826
Organism Klebsiella pneumoniae strain Bckp212

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag HC649_RS18715 Protein ID WP_004142563.1
Coordinates 3748363..3748680 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag HC649_RS18710 Protein ID WP_004142561.1
Coordinates 3748083..3748370 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HC649_RS18680 3744163..3744411 + 249 WP_002893037.1 DUF1158 domain-containing protein -
HC649_RS18685 3744429..3744770 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
HC649_RS18690 3744801..3745916 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
HC649_RS18695 3746096..3746677 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
HC649_RS18700 3746677..3747045 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
HC649_RS18705 3747165..3747818 + 654 WP_094833297.1 oxygen-insensitive NAD(P)H nitroreductase -
HC649_RS18710 3748083..3748370 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
HC649_RS18715 3748363..3748680 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HC649_RS18720 3748865..3749908 - 1044 WP_094833296.1 DUF2157 domain-containing protein -
HC649_RS18725 3750574..3751440 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
HC649_RS18730 3751549..3752976 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T153223 WP_004142563.1 NZ_CP050826:c3748680-3748363 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T153223 NZ_CP066806:3729003-3729545 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTATTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCTGCTAAAGCTCTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAGATTGGTGTTGTCAGATTAGTCATGTTAGGGGTAGCGCG
GAAATATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTAATTTTTTTGAACATGTAAAAATAATTCACCGGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATACTCGCCTCGGCTTTGTTCAGCTTTCAGCG
AGGCCCAATGTTTTCGGTGCTGTACCGATGTTTTTAGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT153223 WP_004142561.1 NZ_CP050826:c3748370-3748083 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT153223 NZ_CP066806:3728708-3729019 [Escherichia coli]
ATGCCTGGAAAAACAGTCGCTCTACCCGATATCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCAGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References