Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 127323..128050 Replicon plasmid pBckp091-1
Accession NZ_CP050823
Organism Klebsiella pneumoniae strain Bckp091

Toxin (Protein)


Gene name higB Uniprot ID A0A7W3D9W1
Locus tag HC645_RS26240 Protein ID WP_011251285.1
Coordinates 127323..127634 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HC645_RS26245 Protein ID WP_011251286.1
Coordinates 127631..128050 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HC645_RS26220 123183..124118 + 936 WP_004026564.1 omptin family outer membrane protease Kop -
HC645_RS26225 124591..125073 + 483 WP_175437602.1 hypothetical protein -
HC645_RS26230 125339..126501 + 1163 WP_088941748.1 IS3 family transposase -
HC645_RS26235 126660..127118 + 459 Protein_130 DDE-type integrase/transposase/recombinase -
HC645_RS26240 127323..127634 + 312 WP_011251285.1 type II toxin-antitoxin system HigB family toxin Toxin
HC645_RS26245 127631..128050 + 420 WP_011251286.1 helix-turn-helix domain-containing protein Antitoxin
HC645_RS26250 128197..129163 + 967 Protein_133 IS5 family transposase -
HC645_RS26255 129307..129993 - 687 Protein_134 hypothetical protein -
HC645_RS26260 129993..130457 - 465 WP_117055985.1 hypothetical protein -
HC645_RS26525 131051..131806 + 756 Protein_136 hypothetical protein -
HC645_RS26270 131999..132523 + 525 WP_029497499.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..157903 157903
- inside IScluster/Tn - - 125339..144503 19164


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12346.12 Da        Isoelectric Point: 9.7248

>T153209 WP_011251285.1 NZ_CP050823:127323-127634 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Download         Length: 312 bp

>T153209 NZ_CP066806:232731-232838 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCCCCGGTCATTGCTGGCATTTTTGCCAGTCTGAT
CGTGAACTCGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15377.46 Da        Isoelectric Point: 4.4420

>AT153209 WP_011251286.1 NZ_CP050823:127631-128050 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT153209 NZ_CP066806:c232674-232619 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3D9W1

Antitoxin

Source ID Structure

References