Detailed information of TA system TA153153

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 204512..204938
• Replicon: plasmid pST45-1
• Accession: NZ_CP050754
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain ST45

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HEC86_RS25490
• Protein ID: WP_001312861.1
• Coordinates: 204512..204670 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	204714..204938 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HEC86_RS25455	199562..199789	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
HEC86_RS25460	199883..200569	-	687	WP_000332484.1	PAS domain-containing protein	-
HEC86_RS25465	200760..201143	-	384	WP_000124981.1	relaxosome protein TraM	-
HEC86_RS25470	201420..202067	+	648	WP_031943493.1	transglycosylase SLT domain-containing protein	-
HEC86_RS25475	202364..203185	-	822	WP_001234469.1	DUF945 domain-containing protein	-
HEC86_RS25480	203303..203590	-	288	WP_000107544.1	hypothetical protein	-
HEC86_RS25490	204512..204670	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	204714..204938	-	225	NuclAT_0	-	Antitoxin
-	204714..204938	-	225	NuclAT_0	-	Antitoxin
-	204714..204938	-	225	NuclAT_0	-	Antitoxin
-	204714..204938	-	225	NuclAT_0	-	Antitoxin
HEC86_RS25495	204950..205669	-	720	WP_013362833.1	plasmid SOS inhibition protein A	-
HEC86_RS25500	205666..206100	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HEC86_RS25505	206155..208113	-	1959	WP_065800285.1	ParB/RepB/Spo0J family partition protein	-
HEC86_RS25510	208179..208412	-	234	WP_000005990.1	DUF905 family protein	-
HEC86_RS25515	208475..209014	-	540	WP_001825195.1	single-stranded DNA-binding protein	-
HEC86_RS25850	209040..209246	-	207	WP_000275858.1	hypothetical protein	-
HEC86_RS25525	209657..209878	-	222	WP_042111886.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / floR / sul2 / aph(4)-Ia / aac(3)-IVa / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(3')-Ia / dfrA12 / oqxA / oqxB / catA2 / blaCTX-M-55	-	1..260432	260432

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T153153 WP_001312861.1 NZ_CP050754:c204670-204512 [Salmonella enterica subsp. enterica serovar Typhimurium]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T153153 NZ_CP066763:c1172138-1171731 [Burkholderia ambifaria]
ATGAAGTTTCTGCTGGATACCAATGCCGTAATCGCCATTCTGAAAGGCGAACAGGCCATGCTGGCCCGATTGCGTAGACA
TCAACCTGCGGATTTCGGAATTCCCGCGATCGTCGCGCATGAACTCTATTACGGGGCGTACAAGAGTCAGAGAGCGGCAG
CGAATCTGGCACGCGTCGAGGGGCTTCAGTTCGAGGTCGTGTCGTTCGATGCGGAAGATGTGCAGCATGCCGGTGAAATC
CGTGCGCAACTGGCCGCGGTGGGAACACCGATCGGGCCATACGATGCATTGATCGCAGGACAGGCGCGAGCGCGACGCCT
GGTGCTTGTCACGCACAACACGCGCGAGTTTGAGCGGGTCCCGGAGCTACAGGTCGAGGATTGGCTCGCGGCGGCGCGCG
AAAAGTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT153153 NZ_CP050754:c204938-204714 [Salmonella enterica subsp. enterica serovar Typhimurium]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin