Detailed information of TA system TA153148

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 204305..204731
• Replicon: plasmid pST46-1
• Accession: NZ_CP050751
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain ST46

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HEC87_RS25490
• Protein ID: WP_001312861.1
• Coordinates: 204305..204463 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	204507..204731 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HEC87_RS25455	199355..199582	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
HEC87_RS25460	199676..200362	-	687	WP_000332484.1	PAS domain-containing protein	-
HEC87_RS25465	200553..200936	-	384	WP_000124981.1	relaxosome protein TraM	-
HEC87_RS25470	201213..201860	+	648	WP_031943493.1	transglycosylase SLT domain-containing protein	-
HEC87_RS25475	202157..202978	-	822	WP_001234469.1	DUF945 domain-containing protein	-
HEC87_RS25480	203096..203383	-	288	WP_000107544.1	hypothetical protein	-
HEC87_RS25490	204305..204463	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	204507..204731	-	225	NuclAT_0	-	Antitoxin
-	204507..204731	-	225	NuclAT_0	-	Antitoxin
-	204507..204731	-	225	NuclAT_0	-	Antitoxin
-	204507..204731	-	225	NuclAT_0	-	Antitoxin
HEC87_RS25495	204743..205462	-	720	WP_013362833.1	plasmid SOS inhibition protein A	-
HEC87_RS25500	205459..205893	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HEC87_RS25505	205948..207906	-	1959	WP_065800285.1	ParB/RepB/Spo0J family partition protein	-
HEC87_RS25510	207972..208205	-	234	WP_000005990.1	DUF905 family protein	-
HEC87_RS25515	208268..208807	-	540	WP_001825195.1	single-stranded DNA-binding protein	-
HEC87_RS25850	208833..209039	-	207	WP_000275858.1	hypothetical protein	-
HEC87_RS25525	209450..209671	-	222	WP_042111886.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / floR / aph(6)-Id / aph(3'')-Ib / sul2 / aph(4)-Ia / aac(3)-IVa / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(3')-Ia / dfrA12 / oqxA / oqxB / catA2 / blaCTX-M-55	-	1..260225	260225

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T153148 WP_001312861.1 NZ_CP050751:c204463-204305 [Salmonella enterica subsp. enterica serovar Typhimurium]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T153148 NZ_CP066763:c250284-250018 [Burkholderia ambifaria]
ATGAGCGTACGCCGCGTGCTCTTCACTTCCGACGCGTGGGACGACTACGTTTATTGGCAAGGGCAAGATCGCAAGACGCT
CAAGCGCATCAACCAGCTCATTCGCGAAGCCCAACGGACGCCGTTCGAAGGCATCGGCAAACCCGAACCACTGAAGGCCA
ATCTGTCCGGTTTCTGGTCACGCCGCATCGACGATACGAACCGTCTCGTCTACGAAGCGGACGATCTTCAGATCAGCATC
GTGTCCTGCCGCTATCACTACGCGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT153148 NZ_CP050751:c204731-204507 [Salmonella enterica subsp. enterica serovar Typhimurium]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin