Detailed information of TA system TA153066

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 41227..41481
• Replicon: plasmid pIncFIB
• Accession: NZ_CP050647
• Organism: Escherichia coli strain PapM-36-2

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: G6008_RS22810
• Protein ID: WP_001553767.1
• Coordinates: 41227..41433 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	41420..41481 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6008_RS22775	37349..37554	-	206	Protein_35	transposase	-
G6008_RS22780	37544..37747	+	204	WP_167778856.1	alkaline phosphatase	-
G6008_RS22785	38035..38322	-	288	WP_089584420.1	type II toxin-antitoxin system RelE/ParE family toxin	-
G6008_RS22790	38319..38570	-	252	WP_001139206.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
G6008_RS22795	39524..40381	-	858	WP_167778857.1	incFII family plasmid replication initiator RepA	-
G6008_RS22800	40374..40448	-	75	WP_001365705.1	RepA leader peptide Tap	-
G6008_RS22805	40695..40943	-	249	WP_000083838.1	replication regulatory protein RepA	-
G6008_RS22810	41227..41433	-	207	WP_001553767.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	41420..41481	+	62	NuclAT_1	-	Antitoxin
-	41420..41481	+	62	NuclAT_1	-	Antitoxin
-	41420..41481	+	62	NuclAT_1	-	Antitoxin
-	41420..41481	+	62	NuclAT_1	-	Antitoxin
G6008_RS22815	42141..42602	-	462	WP_089572206.1	thermonuclease family protein	-
G6008_RS22820	43346..43549	-	204	WP_089571986.1	hypothetical protein	-
G6008_RS22825	43704..44261	-	558	WP_000139319.1	fertility inhibition protein FinO	-
G6008_RS22830	44364..45224	-	861	WP_000868986.1	alpha/beta hydrolase	-
G6008_RS22835	45283..46029	-	747	WP_032285008.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	paa / papC / papH	1..107449	107449

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7804.35 Da
• Isoelectric Point: 8.8807

>T153066 WP_001553767.1 NZ_CP050647:c41433-41227 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGVLAVCATVLCFLLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T153066 NZ_CP066753:c2557266-2556988 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT153066 NZ_CP050647:41420-41481 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin