Detailed information of TA system TA153061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3637062..3637861
• Replicon: chromosome
• Accession: NZ_CP050646
• Organism: Escherichia coli strain PapM-36-2

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F4VJD3
• Locus tag: G6008_RS17400
• Protein ID: WP_000347266.1
• Coordinates: 3637062..3637526 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: G6008_RS17405
• Protein ID: WP_001307405.1
• Coordinates: 3637526..3637861 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6008_RS17370	3632063..3632497	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
G6008_RS17375	3632515..3633393	-	879	WP_001315856.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
G6008_RS17380	3633383..3634162	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
G6008_RS17385	3634173..3634646	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
G6008_RS17390	3634669..3635949	-	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
G6008_RS17395	3636198..3637007	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
G6008_RS17400	3637062..3637526	-	465	WP_000347266.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
G6008_RS17405	3637526..3637861	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
G6008_RS17410	3638010..3639581	-	1572	WP_001273741.1	galactarate dehydratase	-
G6008_RS17415	3639956..3641290	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
G6008_RS17420	3641306..3642076	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17841.18 Da
• Isoelectric Point: 9.4947

>T153061 WP_000347266.1 NZ_CP050646:c3637526-3637062 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T153061 NZ_CP066753:c670289-669825 [Escherichia coli O157:H7]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT153061 WP_001307405.1 NZ_CP050646:c3637861-3637526 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT153061 NZ_CP066753:c670624-670289 [Escherichia coli O157:H7]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A836NGD2

Antitoxin

• AlphaFold DB: A0A0E0XVC7