Detailed information of TA system TA153057

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2851548..2852150
• Replicon: chromosome
• Accession: NZ_CP050646
• Organism: Escherichia coli strain PapM-36-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: G6008_RS13610
• Protein ID: WP_000897305.1
• Coordinates: 2851839..2852150 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G6008_RS13605
• Protein ID: WP_000356397.1
• Coordinates: 2851548..2851838 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6008_RS13580	2847493..2848395	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
G6008_RS13585	2848392..2849027	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
G6008_RS13590	2849024..2849953	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
G6008_RS13595	2850283..2850525	-	243	WP_001087409.1	hypothetical protein	-
G6008_RS13600	2850744..2850962	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
G6008_RS13605	2851548..2851838	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
G6008_RS13610	2851839..2852150	-	312	WP_000897305.1	hypothetical protein	Toxin
G6008_RS13615	2852379..2853287	+	909	WP_001162704.1	alpha/beta hydrolase	-
G6008_RS13620	2853351..2854292	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
G6008_RS13625	2854337..2854774	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
G6008_RS13630	2854771..2855643	-	873	WP_000920762.1	virulence factor BrkB family protein	-
G6008_RS13635	2855637..2856236	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T153057 WP_000897305.1 NZ_CP050646:c2852150-2851839 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T153057 NZ_CP066753:325468-325743 [Escherichia coli O157:H7]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT153057 WP_000356397.1 NZ_CP050646:c2851838-2851548 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT153057 NZ_CP066753:325740-326099 [Escherichia coli O157:H7]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7