Detailed information of TA system TA153009

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3603640..3604300
• Replicon: chromosome
• Accession: NZ_CP050520
• Organism: Enterobacter sp. DNB-S2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HB664_RS17325
• Protein ID: WP_219939884.1
• Coordinates: 3603947..3604300 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1Z3MX79
• Locus tag: HB664_RS17320
• Protein ID: WP_029741460.1
• Coordinates: 3603640..3603942 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB664_RS17290 (HB664_17295)	3598748..3600937	+	2190	WP_045142987.1	TonB-dependent siderophore receptor	-
HB664_RS17300 (HB664_17305)	3601232..3601564	-	333	WP_032658722.1	type II toxin-antitoxin system PemK/MazF family toxin	-
HB664_RS17305 (HB664_17310)	3601565..3601822	-	258	WP_033145928.1	antitoxin	-
HB664_RS17310 (HB664_17315)	3602114..3602503	-	390	WP_219939883.1	RidA family protein	-
HB664_RS17315 (HB664_17320)	3602645..3603565	+	921	WP_126327674.1	LysR family transcriptional regulator	-
HB664_RS17320 (HB664_17325)	3603640..3603942	-	303	WP_029741460.1	XRE family transcriptional regulator	Antitoxin
HB664_RS17325 (HB664_17330)	3603947..3604300	-	354	WP_219939884.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB664_RS17330 (HB664_17335)	3604478..3605371	-	894	WP_219939885.1	LysR substrate-binding domain-containing protein	-
HB664_RS17335 (HB664_17340)	3605545..3606150	+	606	WP_258235895.1	glutathione S-transferase family protein	-
HB664_RS17340 (HB664_17345)	3606194..3607144	-	951	WP_023336219.1	HTH-type transcriptional regulator Cbl	-
HB664_RS17345 (HB664_17350)	3607241..3608158	-	918	WP_033145933.1	nitrogen assimilation transcriptional regulator NAC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13556.41 Da
• Isoelectric Point: 6.9837

>T153009 WP_219939884.1 NZ_CP050520:c3604300-3603947 [Enterobacter sp. DNB-S2]
VWAINTTDRFDRWFTLLDDTERASVLAALLVLREKGPGLSRPYADTLKGSRYSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKVGNERRFYDEMLLVADQEFTHWLDTLKERE

Nucleotide

• Length: 354 bp

>T153009 NZ_CP066719:964702-965064 [Staphylococcus xylosus]
ATGATGAGAAGAGGAGACGTTTATTTAGCTGATTTATCACCAGTACAAGGGTCTGAACAAGGGGGAATTAGACCCGTCGT
TATTATACAAAACGATACTGGTAACAAATATAGTCCTACAGTAATTGTAGCTGCAATTACTGGTAGGATTAATAAAGCTA
AAATACCTACACATGTAGAGATAGAAAAAAGTAAATATAAACTTGATAAAGATTCTGTTATATTGTTAGAACAAATCAGA
ACTTTAGATAAAAATAGGCTAAAGGAAAAATTAACATATCTTTCTGAGAAAAAAATGAAAGAAGTTAATACTGCTATTGA
TATAAGTTTAGGATTACATAATATTAGGAATCATAAATCCTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11050.83 Da
• Isoelectric Point: 5.7316

>AT153009 WP_029741460.1 NZ_CP050520:c3603942-3603640 [Enterobacter sp. DNB-S2]
MGRTLEQLIADEKPEVVASAQAIATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT153009 NZ_CP066719:964535-964705 [Staphylococcus xylosus]
ATGGCAAGTTTTAATCAAAGTAGAAATCATAGTATTGAACAACTTTTAAAAGAAGGCTATTCTCAAATGGCTGACTTAAA
TCTCTCCCTTGCAGCAGAAGCCTTTCCATTTGAATGTGAAGCTTGTGATTGCAATGAAGTTTACATTAGCTCTAAGAATA
ATAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1Z3MX79