Detailed information of TA system TA152978

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1906069..1906729
• Replicon: chromosome
• Accession: NZ_CP050508
• Organism: Raoultella terrigena strain JH01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A485C9M9
• Locus tag: HCK03_RS08920
• Protein ID: WP_041146309.1
• Coordinates: 1906069..1906422 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HCK03_RS08925
• Protein ID: WP_076945586.1
• Coordinates: 1906427..1906729 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCK03_RS08910	1902581..1903012	+	432	WP_115192342.1	universal stress protein	-
HCK03_RS08915	1903063..1905750	+	2688	WP_115192343.1	cation-transporting P-type ATPase	-
HCK03_RS08920	1906069..1906422	+	354	WP_041146309.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HCK03_RS08925	1906427..1906729	+	303	WP_076945586.1	XRE family transcriptional regulator	Antitoxin
HCK03_RS08930	1906970..1908244	-	1275	WP_123551637.1	beta-ketoacyl-ACP synthase II	-
HCK03_RS08935	1908264..1909826	-	1563	WP_041146306.1	DHA2 family efflux MFS transporter permease subunit	-
HCK03_RS08940	1909843..1910866	-	1024	Protein_1742	HlyD family secretion protein	-
HCK03_RS08945	1910850..1911407	-	558	WP_041146304.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (20-89)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13308.30 Da
• Isoelectric Point: 7.5707

>T152978 WP_041146309.1 NZ_CP050508:1906069-1906422 [Raoultella terrigena]
VWIIKTTDRFDGWFSALCATDRACVLALLLVLRERGPGLSRPYADTLKGSCYGNMKELRVQSRGDPIRVFFAFDPCRAGI
LLCAGHKVGNEKRFYEQTIPIADREFTDYLNTLDDKE

Nucleotide

• Length: 354 bp

>T152978 NZ_CP066705:788055-788408 [Staphylococcus pseudintermedius]
ATGAAGCGCGGAGACGTTTATTTAGCAGATTTATCACCAGTACAGGGCTCTGAACAGGGGGGAGTAAGGCCCGTTGTCAT
TATCCAAAATGACACTGGTAATAAGTATAGTCCCACTGTGATTGTAGCCGCAATTACTGGTCGGATTAATAAGGCAAAGA
TACCAACGCATGTCGAAATCGAAAAAAATAAATATAAGCTCGATAAAGATTCTGTTATTTTACTAGAACAAATTCGAACA
GTCGATAAGAAACGATTAAAAGAAAAGTTAACGTTTTTGTCTGAGAAGAAGATGAAAGAAGTTAACAATGCGATTGGTAT
TAGTTTAGGACTGCACATGAATCATCATAAATAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11043.77 Da
• Isoelectric Point: 5.7251

>AT152978 WP_076945586.1 NZ_CP050508:1906427-1906729 [Raoultella terrigena]
MGRTLEQLLADEKPEVVADAQTMATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVASMEKQGRDLKLSTLKRYVEAA
GGKLRLDVELPDGSHFGFAL

Nucleotide

• Length: 303 bp

>AT152978 NZ_CP066705:787884-788054 [Staphylococcus pseudintermedius]
ATGTCAGCATTCAATCAAACAAGATTTAAAAATTTAGAACAGTCGTTAAAAGAAGGTTATGTACAGATGGCAGATTTAAA
TCTCTCCCTAGCCACTGAGGCGTATCCAGTTGAATGTGAAGCTTGCGATTGTAATGAATCACACTTGACAACTGATTACA
AGGAAGACTAA

Structures

Toxin

• AlphaFold DB: A0A485C9M9