Detailed information of TA system TA152963

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1076403..1077078
• Replicon: chromosome
• Accession: NZ_CP050506
• Organism: Enterobacter hormaechei strain CM18-242-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FDC1
• Locus tag: HCU35_RS05150
• Protein ID: WP_015571638.1
• Coordinates: 1076779..1077078 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HCU35_RS05145
• Protein ID: WP_163367520.1
• Coordinates: 1076403..1076768 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCU35_RS05125	1072859..1073590	+	732	WP_017382760.1	methyltransferase domain-containing protein	-
HCU35_RS05130	1073587..1074189	-	603	WP_017382759.1	short chain dehydrogenase	-
HCU35_RS05135	1074287..1075174	+	888	WP_015571641.1	LysR family transcriptional regulator	-
HCU35_RS05140	1075195..1076376	+	1182	WP_017382758.1	PLP-dependent aminotransferase family protein	-
HCU35_RS05145	1076403..1076768	-	366	WP_163367520.1	HigA family addiction module antidote protein	Antitoxin
HCU35_RS05150	1076779..1077078	-	300	WP_015571638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HCU35_RS05155	1077329..1078489	-	1161	WP_085929677.1	HlyD family efflux transporter periplasmic adaptor subunit	-
HCU35_RS05160	1078509..1080701	-	2193	WP_017692801.1	type I secretion system permease/ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11624.29 Da
• Isoelectric Point: 9.6776

>T152963 WP_015571638.1 NZ_CP050506:c1077078-1076779 [Enterobacter hormaechei]
MINHFRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T152963 NZ_CP066667:518177-518518 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCAGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13714.74 Da
• Isoelectric Point: 9.5940

>AT152963 WP_163367520.1 NZ_CP050506:c1076768-1076403 [Enterobacter hormaechei]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTHEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Nucleotide

• Length: 366 bp

>AT152963 NZ_CP066667:517947-518177 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Toxin

• AlphaFold DB: A0A837FDC1