Detailed information of TA system TA152959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 59338..59854
• Replicon: plasmid pLPCN-3
• Accession: NZ_CP050503
• Organism: Lacticaseibacillus paracasei strain NFFJ04

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: HCJ88_RS15690
• Protein ID: WP_193137635.1
• Coordinates: 59573..59854 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: HCJ88_RS15685
• Protein ID: WP_193137634.1
• Coordinates: 59338..59580 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCJ88_RS15675	57022..57942	+	921	WP_193137633.1	IS30 family transposase	-
HCJ88_RS15680	58439..59095	+	657	Protein_58	hypothetical protein	-
HCJ88_RS15685	59338..59580	+	243	WP_193137634.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HCJ88_RS15690	59573..59854	+	282	WP_193137635.1	type II toxin-antitoxin system YafQ family toxin	Toxin
HCJ88_RS15695	59991..60641	+	651	WP_003586220.1	recombinase family protein	-
HCJ88_RS15700	60647..60931	+	285	WP_016363781.1	BRCT domain-containing protein	-
HCJ88_RS15705	61612..61983	+	372	WP_003563277.1	TIGR02328 family protein	-
HCJ88_RS15710	62096..62494	+	399	WP_003563273.1	pyridoxamine 5'-phosphate oxidase family protein	-
HCJ88_RS15715	62968..64047	-	1080	WP_193137636.1	class C sortase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..67740	67740

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (9-92)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-60)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11343.89 Da
• Isoelectric Point: 9.1895

>T152959 WP_193137635.1 NZ_CP050503:59573-59854 [Lacticaseibacillus paracasei]
MNNFIYKPAFERQFKKHYKTILKGGRYKKEDFEAAYHKLLRDEPLDPHYNDHALVNRKPERDLHIKPDWLLIYKYDGEFV
RFIDTGSHSDLFN

Nucleotide

• Length: 282 bp

>T152959 NZ_CP066660:c122654-122382 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACATATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9218.43 Da
• Isoelectric Point: 4.8605

>AT152959 WP_193137634.1 NZ_CP050503:59338-59580 [Lacticaseibacillus paracasei]
MTSQVHFRMSEKEKKDFEIVLSRVGLTPGEAFRIFAKKSIEAGGIPFEVSQPNTRLKKAIRSQDYVEFDDPEEGLDWLNE

Nucleotide

• Length: 243 bp

>AT152959 NZ_CP066660:c122917-122654 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTTGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA