Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 53069..53585 Replicon plasmid pLPCN-2
Accession NZ_CP050502
Organism Lacticaseibacillus paracasei strain NFFJ04

Toxin (Protein)


Gene name yafQ Uniprot ID -
Locus tag HCJ88_RS15295 Protein ID WP_193137581.1
Coordinates 53069..53350 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID -
Locus tag HCJ88_RS15300 Protein ID WP_193137582.1
Coordinates 53343..53585 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCJ88_RS15265 48926..49369 - 444 Protein_55 transposase -
HCJ88_RS15270 49383..50801 - 1419 WP_193137578.1 IS5-like element ISLrh3 family transposase -
HCJ88_RS15275 50937..51098 - 162 Protein_57 transposase -
HCJ88_RS15280 51176..51859 + 684 WP_003586785.1 IS6 family transposase -
HCJ88_RS15285 51991..52275 - 285 WP_193137579.1 BRCT domain-containing protein -
HCJ88_RS15290 52272..52931 - 660 WP_193137580.1 recombinase family protein -
HCJ88_RS15295 53069..53350 - 282 WP_193137581.1 type II toxin-antitoxin system YafQ family toxin Toxin
HCJ88_RS15300 53343..53585 - 243 WP_193137582.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
HCJ88_RS15305 53695..54484 - 790 Protein_63 IS5 family transposase -
HCJ88_RS15310 54483..54841 + 359 Protein_64 hypothetical protein -
HCJ88_RS15315 54964..57567 + 2604 WP_193137583.1 sigma 54-interacting transcriptional regulator -
HCJ88_RS15320 57697..58125 + 429 WP_193137584.1 PTS mannose transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..69836 69836
- flank IS/Tn - - 49383..50801 1418


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(9-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(3-63)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11359.93 Da        Isoelectric Point: 9.5081

>T152958 WP_193137581.1 NZ_CP050502:c53350-53069 [Lacticaseibacillus paracasei]
MNKFIYKPAFERQFKKHYKTILKGGRYKKEDFEAVYHKLLRDEPSDPHYNDHALVNRKPERDLHIKPDWLLIYKYDGEFV
RFIDTGSHSDLFN

Download         Length: 282 bp

>T152958 NZ_CP066659:538460-538801 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9231.47 Da        Isoelectric Point: 5.1548

>AT152958 WP_193137582.1 NZ_CP050502:c53585-53343 [Lacticaseibacillus paracasei]
MTSQVHFRMSEKEKKDFEIVLSRLGLTPGEAFRIFAKKSIEAGGIPFEVSQPNTRLKKAIRSQDYVEFNDPEEGLDWLNE

Download         Length: 243 bp

>AT152958 NZ_CP066659:538230-538460 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References