152950

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4108805..4109604 Replicon chromosome
Accession NZ_CP050498
Organism Escherichia coli strain RM13322

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag HCR07_RS20900 Protein ID WP_000347267.1
Coordinates 4108805..4109269 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HCR07_RS20905 Protein ID WP_001307405.1
Coordinates 4109269..4109604 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCR07_RS20870 4103806..4104240 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
HCR07_RS20875 4104258..4105136 - 879 WP_001298758.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HCR07_RS20880 4105126..4105905 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HCR07_RS20885 4105916..4106389 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HCR07_RS20890 4106412..4107692 - 1281 WP_000681952.1 tagatose-bisphosphate aldolase subunit KbaZ -
HCR07_RS20895 4107941..4108750 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HCR07_RS20900 4108805..4109269 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HCR07_RS20905 4109269..4109604 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HCR07_RS20910 4109753..4111324 - 1572 WP_001273736.1 galactarate dehydratase -
HCR07_RS20915 4111699..4113033 + 1335 WP_000599627.1 galactarate/glucarate/glycerate transporter GarP -
HCR07_RS20920 4113049..4113819 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4108805..4120373 11568


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T152950 WP_000347267.1 NZ_CP050498:c4109269-4108805 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T152950 NZ_CP066645:218356-218622 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT152950 WP_001307405.1 NZ_CP050498:c4109604-4109269 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT152950 NZ_CP066645:218093-218356 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References