Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 3263892..3264494 Replicon chromosome
Accession NZ_CP050498
Organism Escherichia coli strain RM13322

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag HCR07_RS16715 Protein ID WP_000897305.1
Coordinates 3264183..3264494 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HCR07_RS16710 Protein ID WP_000356397.1
Coordinates 3263892..3264182 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCR07_RS16685 3259457..3260092 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
HCR07_RS16690 3260089..3261018 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
HCR07_RS16695 3261417..3262397 + 981 WP_000399648.1 IS110-like element IS621 family transposase -
HCR07_RS16700 3262627..3262869 - 243 WP_001086388.1 hypothetical protein -
HCR07_RS16705 3263088..3263306 - 219 WP_001295676.1 ribbon-helix-helix domain-containing protein -
HCR07_RS16710 3263892..3264182 - 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
HCR07_RS16715 3264183..3264494 - 312 WP_000897305.1 hypothetical protein Toxin
HCR07_RS16720 3264723..3265631 + 909 WP_001162704.1 alpha/beta hydrolase -
HCR07_RS16725 3265695..3266636 - 942 WP_001300148.1 fatty acid biosynthesis protein FabY -
HCR07_RS16730 3266681..3267118 - 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
HCR07_RS16735 3267115..3267987 - 873 WP_000920762.1 virulence factor BrkB family protein -
HCR07_RS16740 3267981..3268580 - 600 WP_001295269.1 glucose-1-phosphatase -
HCR07_RS16745 3268679..3269464 - 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3261417..3262397 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T152948 WP_000897305.1 NZ_CP050498:c3264494-3264183 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T152948 NZ_CP066640:28588-28710 [Enterococcus faecium]
ATGAAAGGAGTAAGCCTTTTGTCCGCATATGAGACAATTCAAACCATTCTTGGTTTTGGTATGTTTACCATTGCTTTGAT
TAGTTTGATTGTAAATATGCTTAAAAATGACAAAAAGAAATAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT152948 WP_000356397.1 NZ_CP050498:c3264182-3263892 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT152948 NZ_CP066640:c28793-28692 [Enterococcus faecium]
CCCTCATTAACAGCACCGTTTAAAGAACGGCGGCTCAGATTAATTATTTATTGAGAAATAACCGATCCTCGCTAAAGTGA
CGGTTATTTCTTTTTGTCATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References