152947

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2980147..2980644 Replicon chromosome
Accession NZ_CP050498
Organism Escherichia coli strain RM13322

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HCR07_RS15475 Protein ID WP_001173343.1
Coordinates 2980147..2980320 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HCR07_RS15480 Protein ID WP_000398619.1
Coordinates 2980348..2980644 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCR07_RS15465 2976817..2978274 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
HCR07_RS15470 2978511..2980028 + 1518 WP_001295074.1 lysine--tRNA ligase -
HCR07_RS15475 2980147..2980320 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HCR07_RS15480 2980348..2980644 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HCR07_RS15485 2980871..2981143 - 273 WP_000405647.1 N-acetyltransferase -
HCR07_RS15490 2981155..2981385 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
HCR07_RS15495 2981566..2983197 + 1632 WP_001216487.1 sensor histidine kinase -
HCR07_RS15500 2983194..2983913 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2971293..2980644 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T152947 WP_001173343.1 NZ_CP050498:c2980320-2980147 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T152947 NZ_CP066637:c1651254-1651072 [Enterococcus faecium]
ATGCCAATGACGCAAAATGAAATGGTGAAACTCCTAATTGAACACGGCGGCGTTAAAGTCAAAGGTGGGAAAGGATCTCA
TATAAAAGTGAGGTTTCCTGGAGTGAACCGGCCGATCATTGTTCCTCAGAAATTACCCAAAGGGACAGAAAACGGTATTT
TGAAGCAAGCAGGGTTGAAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT152947 WP_000398619.1 NZ_CP050498:c2980644-2980348 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT152947 NZ_CP066637:c1651034-1650579 [Enterococcus faecium]
ATGCTTGTTTCTTATCCGGCCTTGTTCTATTTCGAAAATAGCGAAGGCTATGACCCTGGATTTTCTGTTTTCTTTCCAGA
CTTTCCCAATACAGGTGGTACGAGTGGTTCTACAATCTCTGAAGCTCTGGAAATGGCGTCTGATTATTTAGGAATTTTAT
TATCTGATGATATTGAACAGGAACGCGAGTTACCAAAACCTTCCTTAATCAGTTCGCTATCTTTAGAAGAGAATGATCCT
TTTAAAGATGATTCAGATTTTAATTTTGAATATGATTTTGAAAAATCGTTTGTGTCTATGGTGAGTGTTGATCTATCCCA
ATATCTTGGGAATAATGATCCCATTAAAAAGACTTTAACTATTCCCAAGTGGGCAGATAAGCTTGGAAAGGATCTTCATT
TGAACTTTTCAAAGACTTTAACTGATGCAATCGTAAGCAAAAAAATGGGTGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References