Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2354020..2354561 Replicon chromosome
Accession NZ_CP050498
Organism Escherichia coli strain RM13322

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag HCR07_RS12535 Protein ID WP_000615976.1
Coordinates 2354283..2354561 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HCR07_RS12530 Protein ID WP_000729704.1
Coordinates 2354020..2354280 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCR07_RS12505 2349803..2350588 - 786 WP_000207567.1 putative lateral flagellar export/assembly protein LafU -
HCR07_RS12510 2350533..2352272 + 1740 WP_001298878.1 flagellar biosynthesis protein FlhA -
HCR07_RS12515 2352377..2352655 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
HCR07_RS12520 2352648..2353004 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
HCR07_RS12525 2353061..2353834 - 774 WP_000543899.1 C40 family peptidase -
HCR07_RS12530 2354020..2354280 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HCR07_RS12535 2354283..2354561 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HCR07_RS12540 2354717..2355457 + 741 WP_001225679.1 murein L,D-transpeptidase -
HCR07_RS12545 2355428..2356195 - 768 WP_000333380.1 class II glutamine amidotransferase -
HCR07_RS12550 2356401..2356979 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T152943 WP_000615976.1 NZ_CP050498:2354283-2354561 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T152943 NZ_CP066629:521186-521527 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCAGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT152943 WP_000729704.1 NZ_CP050498:2354020-2354280 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT152943 NZ_CP066629:520956-521186 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References