Detailed information of TA system TA152918

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 2527352..2527792
• Replicon: chromosome
• Accession: NZ_CP050491
• Organism: Enterococcus faecalis strain EGM183

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: A0A4U4MH53
• Locus tag: EGM183_RS12060
• Protein ID: WP_073340330.1
• Coordinates: 2527352..2527504 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	2527596..2527792 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGM183_RS12045	2522645..2523358	-	714	WP_002367455.1	trehalose operon repressor	-
EGM183_RS12050	2523620..2526364	+	2745	WP_174097082.1	glycoside hydrolase family 65 protein	-
EGM183_RS12055	2526379..2527029	+	651	WP_002372772.1	beta-phosphoglucomutase	-
-	2527098..2527157	+	60	NuclAT_13	-	-
-	2527232..2527419	+	188	NuclAT_7	-	-
EGM183_RS12060	2527352..2527504	-	153	WP_073340330.1	putative holin-like toxin	Toxin
-	2527596..2527792	+	197	NuclAT_8	-	Antitoxin
EGM183_RS12065	2527725..2527868	-	144	WP_170965521.1	putative holin-like toxin	-
EGM183_RS12070	2528100..2529071	-	972	WP_002381060.1	ribose-phosphate diphosphokinase	-
EGM183_RS12075	2529246..2529683	-	438	WP_192177094.1	peptide-methionine (R)-S-oxide reductase MsrB	-
EGM183_RS12080	2529816..2530370	-	555	WP_002354869.1	septum formation protein Maf	-
EGM183_RS12085	2530395..2532527	-	2133	WP_104886135.1	DNA mismatch repair endonuclease MutL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (17-50)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5665.96 Da
• Isoelectric Point: 11.0942

>T152918 WP_073340330.1 NZ_CP050491:c2527504-2527352 [Enterococcus faecalis]
VVRMSVLPKFTERRGLLSAYETIQTILGFGMFTIALIALIVKLLKNDKKK

Nucleotide

• Length: 153 bp

>T152918 NZ_CP066583:210167-210433 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Nucleotide

• Length: 197 bp

>AT152918 NZ_CP050491:2527596-2527792 [Enterococcus faecalis]
TTTTTAGGGAAATGTGCTATAATAAAAATGAAAAGAGAGATATGCTTCAACATACCTCTCTGATGCAGAGCCGTTTAAGA
CGGTGACCAATTTAGTTATAAAAATAACCGTACCGGTCAAAGTTGACGGTTATTTTTTATTGTCATTTTTAAGCAATTTC
ACAATCAGCGCAATCGAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: A0A4U4MH53

Antitoxin