Detailed information of TA system TA152874

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5562..6213
• Replicon: plasmid pPM194229_3
• Accession: NZ_CP050435
• Organism: Acinetobacter baumannii strain PM194229

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: HB663_RS20110
• Protein ID: WP_000269904.1
• Coordinates: 5562..5918 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HB663_RS20115
• Protein ID: WP_001129974.1
• Coordinates: 5911..6213 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB663_RS20095	992..1537	+	546	WP_000736775.1	plasmid replication DNA-binding protein	-
HB663_RS20100	1744..4416	-	2673	WP_148307306.1	ATP-binding protein	-
HB663_RS20105	4556..5023	+	468	WP_079393076.1	DUF1643 domain-containing protein	-
HB663_RS20110	5562..5918	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB663_RS20115	5911..6213	+	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
HB663_RS20120	6206..6415	+	210	WP_000069475.1	hypothetical protein	-
HB663_RS20125	6590..7108	+	519	WP_000447195.1	sel1 repeat family protein	-
HB663_RS20130	7147..7659	+	513	WP_001089574.1	SMI1/KNR4 family protein	-
HB663_RS20135	7766..8083	-	318	WP_000900860.1	hypothetical protein	-
HB663_RS20140	8288..8479	-	192	WP_000102616.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..9847	9847

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T152874 WP_000269904.1 NZ_CP050435:5562-5918 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T152874 NZ_CP066552:c4679518-4678241 [Pectobacterium carotovorum]
ATGGCTGCGCTCGATGTTTACATGAACGGCTATCGGGTGGGGATTCTGACCAAAACGGGCAGTGGAGCACATCATTTTTC
CTATGATGCGAACTGGCTCGGGTTGCCGGGGAGTCGCCCCATTTCGCTTTCTGTGCCGCTACGTCATCAGTCCTATCAAG
GTGATGAGGTTTATAATTTCTTCGACAATTTGTTGCCGGATAACCCGGATATCAGAAGACGGATTGTCGCCAGGTATCAC
ACGGATTCCACCCAACCGTTTGATCTGCTGGCTAAAGTGGGGCAAGACAGTGTCGGGGCGTTACAATTAGTGCCGCAGGG
TTCACCTGTGCACGATATAAAAAAGATCGAATACAAAACCTTATCCGAGCAGCAGCTTGAAAACATACTGACCGGATATC
TGTACGATGCCCCGCTGGGTATGATTGACACTGAAGACGATTTTCGTATTTCTATCGCAGGTGCACAGGAAAAGACGGCG
CTGCTTTATCTGGATGATCACTGGTGCCTGCCTCTCAATGCCACACCAACTACCCACATCATCAAGCTACCGATTGGTAA
AATCGAGAGTCACTCTTACTCAATTGATATGTCTGACAGCGTTGAAAATGAGTATCTGTGCCTGCTCATTGCCAAGGCAT
TTGGGCTGCCAGTGCCGCACTGTTTTATGATCAAAGCTGGCAAGATAAAGGCGCTGGCGGTGGAGCGTTTTGATCGTAAA
TACGCCTCGGATGTCAGTTGGATTATGCGGCTACCACAAGAGGATTTTTGTCAGGTATTAAACGTTCCTTCCGCACTGAA
ATATGAAAATCATGGTGGACCGGGTATTTCCGCCATTATGTCGTTTCTATTGGGTTCGGTTGATCCAGAAAGAGATCGTT
ATCGCTTTATGCAGGCTCAGGTACTGTTTTGGCTATTGGCTGCAACGGATGGTCATGCGAAAAATTTTTCCTTGTTTATT
GAATCTGAGGGGCGATATCGACTCACGCCTTTCTATGACATTCTCTCTATGTATCCGGCGATGGGATTAACTGGCTCAAG
AGGCAAGAAATATGCGATTGAGCAGATCTTTCCCCGGCATTTTTTCCAGACGGCGAAGGCCGTTGGGTTTGCCAGAGAAT
CAATGGAAAACATTCTGGCGGAGTTTGCACAAACCGTTGATAACGTCATTACGACTGTTGGAAGTCAACTGCCAGTGGAT
TTTCCTACTCATATCAGTGAGGCAATCCTGACCGGGTTGCTGGCTAGATCGAGACGGCTCATGAGAGGATGGGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT152874 WP_001129974.1 NZ_CP050435:5911-6213 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT152874 NZ_CP066552:c4679784-4679518 [Pectobacterium carotovorum]
ATGAAGGTCACCAACAGCAAGCAGCTTAGCAGCTATCTGAAAGATGTTCGTATCACGCAGAAGCTTTCACAAGGGAAAGT
GGCCAGTAAAGTGGGTATTCGCCAGGATACGGTATCCAGTTTCGAGCAACATCCTGATTCCACTAAGCTGGAAACCTTCT
TTAAAATCCTGTCGGCATTGAATTTAGAACTTACTGTTACGCCCCGGAATGTGGACACCACCAATAACGAAGCCTCTGTT
GCATCATCCTGGAAGGAAGAATGGTAA

Structures

Toxin

• AlphaFold DB: N8YFZ1