Detailed information of TA system TA152868

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2754..3405
• Replicon: plasmid pPM194122_4
• Accession: NZ_CP050429
• Organism: Acinetobacter baumannii strain PM194188

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4R0DYU1
• Locus tag: HB368_RS20565
• Protein ID: WP_000269907.1
• Coordinates: 2754..3110 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HB368_RS20570
• Protein ID: WP_001140617.1
• Coordinates: 3103..3405 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB368_RS20545	1..951	+	951	WP_001205343.1	replication initiation protein RepM	-
HB368_RS20550	944..1519	+	576	WP_169059681.1	helix-turn-helix domain-containing protein	-
HB368_RS20555	1539..1682	+	144	WP_169059682.1	hypothetical protein	-
HB368_RS20565	2754..3110	+	357	WP_000269907.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB368_RS20570	3103..3405	+	303	WP_001140617.1	helix-turn-helix transcriptional regulator	Antitoxin
HB368_RS20575	3528..3929	+	402	WP_000660211.1	hypothetical protein	-
HB368_RS20580	3946..4248	+	303	WP_001081086.1	hypothetical protein	-
HB368_RS20585	4313..5053	-	741	WP_031380848.1	DUF3800 domain-containing protein	-
HB368_RS20590	5364..5750	+	387	WP_001986792.1	helix-turn-helix domain-containing protein	-
HB368_RS20595	5787..5960	-	174	WP_001282484.1	hypothetical protein	-
HB368_RS20600	5960..6331	-	372	WP_000504218.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..7540	7540

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7172

>T152868 WP_000269907.1 NZ_CP050429:2754-3110 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYTEMLAIADQQYELHLSTLGEQSNG

Nucleotide

• Length: 357 bp

>T152868 NZ_CP066552:3213061-3213375 [Pectobacterium carotovorum]
ATGCAATTCACTGTATACGGTAATACCGGGAAAAGCGTCGTTTACCCGCTGTTGCTCGATGTCACGAGCGATATTATCGG
GCAATTGAATCGTCGGATAGTGATCCCATTGCTCCCTATTGAAAAGTATCCGGCAGGCCGCCGACCGGATCGCCTTATCC
CCGTCGTCAGGCTGACGGATGGCAAAGAATACGCCGTAATGACCCACGAACTGGCAAGTATCCCTGTCCAGGCACTGGGG
GCGGTGTTTTGTGATGCTTCGCAGTACCGCACTCAGGTAAAGGCGGCAATAGACTTCCTCATCGACGGGTTCTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11262.05 Da
• Isoelectric Point: 5.8961

>AT152868 WP_001140617.1 NZ_CP050429:3103-3405 [Acinetobacter baumannii]
MAKTLQELLANRSPESQARIQKIADELLLENQLYLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT152868 NZ_CP066552:3212822-3213058 [Pectobacterium carotovorum]
ATGACCGCGAAACAGCGCAACACGCAGAGCGTGACCATGACAGTAGAGCGTGCCTTACTGGTAAGAGCGCGTGAAGCGGG
TATTAATCTGAGTGCTACCCTGACAACCGCCCTGGATGCAGAGCTTCGCTATCATGAAGCGAAAAAATGGCAGGAAGAAA
ACAGGGAGGCTCTCGCAGCACTAAATCATTTTCATGATGAGCAGGGGTGTTTCAGCGATGAATACAGGGCGTTTTAA

Structures

Toxin

• AlphaFold DB: A0A4R0DYU1