Detailed information of TA system TA152866

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 15068..15653
• Replicon: plasmid pPM194122_2
• Accession: NZ_CP050427
• Organism: Acinetobacter baumannii strain PM194188

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8ZPD7
• Locus tag: HB368_RS20460
• Protein ID: WP_000897309.1
• Coordinates: 15333..15653 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HB368_RS20455
• Protein ID: WP_053502713.1
• Coordinates: 15068..15340 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB368_RS20440	10344..10919	-	576	WP_001052939.1	biliverdin-producing heme oxygenase	-
HB368_RS20445	11035..13815	-	2781	WP_031380699.1	TonB-dependent hemoglobin/transferrin/lactoferrin family receptor	-
HB368_RS20450	13743..14198	-	456	WP_001187712.1	YbaN family protein	-
HB368_RS20455	15068..15340	-	273	WP_053502713.1	helix-turn-helix domain-containing protein	Antitoxin
HB368_RS20460	15333..15653	-	321	WP_000897309.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB368_RS20465	15840..16037	-	198	WP_053502715.1	hypothetical protein	-
HB368_RS20470	16104..16331	-	228	WP_004745640.1	hypothetical protein	-
HB368_RS20475	16429..16644	-	216	WP_000071892.1	cold-shock protein	-
HB368_RS20480	16976..17149	-	174	WP_001282484.1	hypothetical protein	-
HB368_RS20485	17149..17520	-	372	WP_000504218.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..18783	18783

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12209.01 Da
• Isoelectric Point: 7.9835

>T152866 WP_000897309.1 NZ_CP050427:c15653-15333 [Acinetobacter baumannii]
MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T152866 NZ_CP066552:1760819-1761172 [Pectobacterium carotovorum]
GTGTGGAGAATTGAAACGACGGACGTGTTTGATAGCTGGTTCAGCGCCTTAAACGATAGGGATCGAGCTTGCGTGCTTGC
AGCACTTATCGTGTTGCGGGAAAAAGGTCCAATGTTATCCAGACCTTATGCCGATACGGTTAAAGGTTCTCGCTATAGCA
ACATGAAAGAGCTGCGTATTCAGAGCCGAGGAACGCCGATACGGGCATTTTTTGCCTTCGACCCACATCGTACAGGGATC
CTGCTCTGCGCGGGCAATAAGTCCGGTAATGAAAAGCGCTTTTACGATGAGATGCTCCCGTTTGCTGAGCGGGAGTTAAC
AAATTATCTGAAAAAATCAGAGAAGAGGGAGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10091.53 Da
• Isoelectric Point: 8.7029

>AT152866 WP_053502713.1 NZ_CP050427:c15340-15068 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRNEIKASRVTALELPDIKEVRERTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDAHPNLI

Nucleotide

• Length: 273 bp

>AT152866 NZ_CP066552:1761177-1761479 [Pectobacterium carotovorum]
ATGGGCAGAACACTGGAACAGCTTCTTGCGGATGAAAAGCCAGAGGTCGTGGTCGATGCTCACGCTATAGCGACAGAGAT
GCTGCTCAACATTCATCTCGCCGAACTGCGTGAGAGGGTGCAAAAAACGCAAGTAGAAATGGCGCAGGTGCTTGGGTTAA
AACAGCCGACCGTGGCGGTAATGGAGAAACCCGGTCGCGATCTGAAACTTTCCACGCTTAAACGCTATGTCGAAGCGACT
GGCAGTAAGCTACGTCTTGATGTTGAACTACCGGACGGCTCGCATTACGAATTTATGCTGTAA

Structures

Toxin

• AlphaFold DB: A0A855WJQ3