Detailed information of TA system TA152846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 22236..22894
• Replicon: plasmid pPM192696_1
• Accession: NZ_CP050413
• Organism: Acinetobacter baumannii strain PM192696

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HB366_RS19180
• Protein ID: WP_000312250.1
• Coordinates: 22535..22894 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HB366_RS19175
• Protein ID: WP_001096429.1
• Coordinates: 22236..22535 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB366_RS19135	17261..18541	+	1281	WP_001093570.1	hypothetical protein	-
HB366_RS19140	18646..18903	+	258	WP_000834290.1	hypothetical protein	-
HB366_RS19145	18908..19480	+	573	WP_000443897.1	hypothetical protein	-
HB366_RS19150	19456..19635	+	180	WP_000387630.1	hypothetical protein	-
HB366_RS19155	19652..20281	+	630	WP_000701003.1	hypothetical protein	-
HB366_RS19160	20321..20539	+	219	WP_001043201.1	hypothetical protein	-
HB366_RS19165	20559..21113	+	555	WP_000790084.1	hypothetical protein	-
HB366_RS19170	21163..21699	+	537	WP_000731978.1	hypothetical protein	-
HB366_RS19175	22236..22535	-	300	WP_001096429.1	helix-turn-helix transcriptional regulator	Antitoxin
HB366_RS19180	22535..22894	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB366_RS19185	23095..23661	+	567	WP_000710385.1	hypothetical protein	-
HB366_RS19190	23710..23892	+	183	WP_000373385.1	hypothetical protein	-
HB366_RS19195	23959..24657	+	699	WP_000873188.1	hypothetical protein	-
HB366_RS19200	24796..25179	+	384	WP_000654348.1	hypothetical protein	-
HB366_RS19205	25246..26004	+	759	WP_001053127.1	hypothetical protein	-
HB366_RS19210	27059..27373	+	315	WP_000708714.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-VIa	-	1..70098	70098

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T152846 WP_000312250.1 NZ_CP050413:c22894-22535 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T152846 NZ_CP066543:5132101-5132484 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT152846 WP_001096429.1 NZ_CP050413:c22535-22236 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT152846 NZ_CP066543:5131859-5132101 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA