Detailed information of TA system TA152842

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 4888..5473
• Replicon: plasmid pVB2486_3
• Accession: NZ_CP050406
• Organism: Acinetobacter baumannii strain VB2486

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: HBN34_RS20670
• Protein ID: WP_000897307.1
• Coordinates: 5153..5473 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HBN34_RS20665
• Protein ID: WP_000369781.1
• Coordinates: 4888..5160 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBN34_RS20645	603..935	-	333	WP_004282109.1	hypothetical protein	-
HBN34_RS20650	1179..1790	+	612	WP_015060246.1	recombinase family protein	-
HBN34_RS20655	1980..2864	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
HBN34_RS20660	2920..4395	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
HBN34_RS20665	4888..5160	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
HBN34_RS20670	5153..5473	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HBN34_RS20675	5682..6119	-	438	WP_031985418.1	hypothetical protein	-
HBN34_RS20680	6112..7491	-	1380	WP_031985417.1	ATP-binding protein	-
HBN34_RS20685	7786..8403	-	618	WP_120752772.1	plasmid replication DNA-binding protein	-
HBN34_RS20690	8480..9379	-	900	WP_004763517.1	replication initiation protein RepM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(E) / msr(E)	-	1..12574	12574

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T152842 WP_000897307.1 NZ_CP050406:c5473-5153 [Acinetobacter baumannii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T152842 NZ_CP066543:c4175222-4174815 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT152842 WP_000369781.1 NZ_CP050406:c5160-4888 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT152842 NZ_CP066543:c4175469-4175203 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3