Detailed information of TA system TA152822

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 76177..76762
• Replicon: plasmid pVB82_1
• Accession: NZ_CP050386
• Organism: Acinetobacter baumannii strain VB82

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7S7BY50
• Locus tag: HBK86_RS20770
• Protein ID: WP_000897311.1
• Coordinates: 76442..76762 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HBK86_RS20765
• Protein ID: WP_000369784.1
• Coordinates: 76177..76449 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBK86_RS20730	72044..72223	+	180	Protein_67	IS6 family transposase	-
HBK86_RS20735	72274..72978	-	705	WP_001067788.1	IS6-like element IS1008 family transposase	-
HBK86_RS20740	73043..73444	+	402	Protein_69	hydroxyacid dehydrogenase	-
HBK86_RS20745	73441..74298	+	858	WP_000609033.1	LysR family transcriptional regulator	-
HBK86_RS20750	74624..74836	-	213	WP_001123161.1	hypothetical protein	-
HBK86_RS20755	74952..75253	-	302	Protein_72	transposase	-
HBK86_RS20760	75344..76144	-	801	WP_000604996.1	DUF2971 domain-containing protein	-
HBK86_RS20765	76177..76449	-	273	WP_000369784.1	helix-turn-helix domain-containing protein	Antitoxin
HBK86_RS20770	76442..76762	-	321	WP_000897311.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HBK86_RS20775	77441..78373	-	933	WP_000743272.1	IS5-like element IS17 family transposase	-
HBK86_RS20780	78427..78864	+	438	Protein_77	IS3 family transposase	-
HBK86_RS20785	79122..80354	+	1233	WP_001255535.1	ATP-binding protein	-
HBK86_RS20795	80626..80955	-	330	WP_000506885.1	DUF4236 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-Ian / blaOXA-23 / aph(3'')-Ib / aph(6)-Id / sul2 / tet(B) / mph(E) / msr(E) / armA / sul1 / blaPER-7 / qacE / cmlA1 / ARR-3	-	1..215278	215278
	-	inside	IScluster/Tn	aac(6')-Ian	-	67553..78864	11311

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-84)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12214.03 Da
• Isoelectric Point: 7.9500

>T152822 WP_000897311.1 NZ_CP050386:c76762-76442 [Acinetobacter baumannii]
MLFIETSIFTKQIKELVTDEEYRQLQQDLLIQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFNG

Nucleotide

• Length: 321 bp

>T152822 NZ_CP066534:c1435910-1435692 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10145.57 Da
• Isoelectric Point: 7.0146

>AT152822 WP_000369784.1 NZ_CP050386:c76449-76177 [Acinetobacter baumannii]
MDNNLFDDLVTSIKEAGAIKRNEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDTNPTLI

Nucleotide

• Length: 273 bp

>AT152822 NZ_CP066534:c1436311-1435937 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A7S7BY50