Detailed information of TA system TA152816

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 16009..16263
• Replicon: plasmid p52148_NDM_5
• Accession: NZ_CP050384
• Organism: Escherichia coli strain 52148

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: HB638_RS23410
• Protein ID: WP_032215358.1
• Coordinates: 16009..16215 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	16202..16263 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB638_RS23380	12032..12433	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
HB638_RS23385	12366..12623	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
HB638_RS23390	12716..13369	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
HB638_RS23395	14308..15165	-	858	WP_029702152.1	incFII family plasmid replication initiator RepA	-
HB638_RS23400	15158..15232	-	75	WP_001365705.1	RepA leader peptide Tap	-
HB638_RS23405	15468..15725	-	258	WP_000083833.1	replication regulatory protein RepA	-
HB638_RS23410	16009..16215	-	207	WP_032215358.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	16202..16263	+	62	NuclAT_1	-	Antitoxin
-	16202..16263	+	62	NuclAT_1	-	Antitoxin
-	16202..16263	+	62	NuclAT_1	-	Antitoxin
-	16202..16263	+	62	NuclAT_1	-	Antitoxin
HB638_RS24100	16402..16668	-	267	WP_087757657.1	hypothetical protein	-
HB638_RS23420	17043..17612	-	570	WP_029702150.1	DUF2726 domain-containing protein	-
HB638_RS23425	17764..18390	-	627	WP_029702149.1	hypothetical protein	-
HB638_RS23430	18802..19011	-	210	WP_039023209.1	hypothetical protein	-
HB638_RS23435	19142..19702	-	561	WP_001567328.1	fertility inhibition protein FinO	-
HB638_RS23440	19757..20503	-	747	WP_052273601.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / mph(A) / blaNDM-5 / sul1 / qacE / aadA2	-	1..121872	121872
	-	inside	Integron	qacE / dfrA12 / aadA2	-	2..121837	121835

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7778.27 Da
• Isoelectric Point: 8.8807

>T152816 WP_032215358.1 NZ_CP050384:c16215-16009 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T152816 NZ_CP066533:c3337-3071 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT152816 NZ_CP050384:16202-16263 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin