Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | DinJ-YafQ (relBE)/YafQ-RelB |
| Location | 4283246..4283787 | Replicon | chromosome |
| Accession | NZ_CP050382 | ||
| Organism | Escherichia coli strain 52148 | ||
Toxin (Protein)
| Gene name | yafQ | Uniprot ID | Q47149 |
| Locus tag | HB638_RS20565 | Protein ID | WP_000615983.1 |
| Coordinates | 4283509..4283787 (+) | Length | 93 a.a. |
Antitoxin (Protein)
| Gene name | dinJ | Uniprot ID | Q47150 |
| Locus tag | HB638_RS20560 | Protein ID | WP_000729703.1 |
| Coordinates | 4283246..4283506 (+) | Length | 87 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HB638_RS20540 | 4278921..4279706 | - | 786 | WP_000207552.1 | putative lateral flagellar export/assembly protein LafU | - |
| HB638_RS20545 | 4279678..4281390 | + | 1713 | Protein_4017 | flagellar biosynthesis protein FlhA | - |
| HB638_RS20550 | 4281614..4282111 | - | 498 | WP_000006255.1 | REP-associated tyrosine transposase RayT | - |
| HB638_RS20555 | 4282287..4283036 | - | 750 | WP_000056849.1 | C40 family peptidase | - |
| HB638_RS20560 | 4283246..4283506 | + | 261 | WP_000729703.1 | type II toxin-antitoxin system antitoxin DinJ | Antitoxin |
| HB638_RS20565 | 4283509..4283787 | + | 279 | WP_000615983.1 | type II toxin-antitoxin system mRNA interferase toxin YafQ | Toxin |
| HB638_RS20570 | 4283943..4284683 | + | 741 | WP_001225679.1 | murein L,D-transpeptidase | - |
| HB638_RS20575 | 4284654..4285421 | - | 768 | WP_000333380.1 | class II glutamine amidotransferase | - |
| HB638_RS20580 | 4285627..4286205 | - | 579 | WP_000284050.1 | D-sedoheptulose 7-phosphate isomerase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 93 a.a. Molecular weight: 10846.62 Da Isoelectric Point: 9.9941
>T152812 WP_000615983.1 NZ_CP050382:4283509-4283787 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG
Download Length: 279 bp
>T152812 NZ_CP066529:c47839-47681 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG
Antitoxin
Download Length: 87 a.a. Molecular weight: 9405.80 Da Isoelectric Point: 5.0758
>AT152812 WP_000729703.1 NZ_CP050382:4283246-4283506 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI
Download Length: 261 bp
>AT152812 NZ_CP066529:c48107-47883 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|