152811

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4277049..4277743 Replicon chromosome
Accession NZ_CP050382
Organism Escherichia coli strain 52148

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag HB638_RS20525 Protein ID WP_001263489.1
Coordinates 4277049..4277447 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag HB638_RS20530 Protein ID WP_000554758.1
Coordinates 4277450..4277743 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 4272637..4272717 - 81 NuclAT_13 - -
- 4272637..4272717 - 81 NuclAT_13 - -
- 4272637..4272717 - 81 NuclAT_13 - -
- 4272637..4272717 - 81 NuclAT_13 - -
HB638_RS20500 4273313..4273771 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
HB638_RS20505 4274032..4275489 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
HB638_RS20510 4275546..4276067 - 522 Protein_4010 peptide chain release factor H -
HB638_RS20515 4276063..4276269 - 207 Protein_4011 RtcB family protein -
HB638_RS20520 4276587..4277039 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
HB638_RS20525 4277049..4277447 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HB638_RS20530 4277450..4277743 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HB638_RS20535 4277795..4278850 - 1056 WP_001226164.1 DNA polymerase IV -
HB638_RS20540 4278921..4279706 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
HB638_RS20545 4279678..4281390 + 1713 Protein_4017 flagellar biosynthesis protein FlhA -
HB638_RS20550 4281614..4282111 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T152811 WP_001263489.1 NZ_CP050382:c4277447-4277049 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T152811 NZ_CP066529:7252-7668 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT152811 WP_000554758.1 NZ_CP050382:c4277743-4277450 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT152811 NZ_CP066529:7025-7255 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References