Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1155263..1156062 Replicon chromosome
Accession NZ_CP050382
Organism Escherichia coli strain 52148

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag HB638_RS05510 Protein ID WP_000347275.1
Coordinates 1155263..1155727 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HB638_RS05515 Protein ID WP_001307405.1
Coordinates 1155727..1156062 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB638_RS05480 1150264..1150698 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
HB638_RS05485 1150716..1151594 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HB638_RS05490 1151584..1152363 - 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HB638_RS05495 1152374..1152847 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
HB638_RS05500 1152870..1154150 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
HB638_RS05505 1154399..1155208 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HB638_RS05510 1155263..1155727 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HB638_RS05515 1155727..1156062 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HB638_RS05520 1156211..1157782 - 1572 WP_001273752.1 galactarate dehydratase -
HB638_RS05525 1158157..1159491 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HB638_RS05530 1159507..1160277 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T152791 WP_000347275.1 NZ_CP050382:c1155727-1155263 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T152791 NZ_CP066524:31072-31230 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT152791 WP_001307405.1 NZ_CP050382:c1156062-1155727 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT152791 NZ_CP066524:30961-31026 [Klebsiella pneumoniae]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References