Detailed information of TA system TA152754

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 66216..66485
• Replicon: plasmid p50595_ERM
• Accession: NZ_CP050372
• Organism: Klebsiella pneumoniae strain 50595

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HB637_RS27280
• Protein ID: WP_001312861.1
• Coordinates: 66327..66485 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	66216..66281 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB637_RS27255	61985..62512	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
HB637_RS27260	62570..62803	+	234	WP_000006003.1	DUF905 family protein	-
HB637_RS27265	62864..64828	+	1965	WP_063131970.1	ParB/RepB/Spo0J family partition protein	-
HB637_RS27270	64897..65331	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HB637_RS27275	65328..66047	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	66059..66283	+	225	NuclAT_0	-	-
-	66059..66283	+	225	NuclAT_0	-	-
-	66059..66283	+	225	NuclAT_0	-	-
-	66059..66283	+	225	NuclAT_0	-	-
-	66216..66281	+	66	-	-	Antitoxin
HB637_RS27280	66327..66485	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
HB637_RS29915	66723..67100	-	378	Protein_79	hypothetical protein	-
HB637_RS27295	67400..67696	+	297	WP_001272251.1	hypothetical protein	-
HB637_RS27300	67807..68628	+	822	WP_001234445.1	DUF945 domain-containing protein	-
HB637_RS27305	68925..69527	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
HB637_RS27310	69850..70233	+	384	WP_001354030.1	relaxosome protein TraM	-
HB637_RS27315	70427..71098	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
HB637_RS27320	71235..71462	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / mph(A)	-	1..76387	76387

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T152754 WP_001312861.1 NZ_CP050372:66327-66485 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T152754 NZ_CP066492:c1972444-1972292 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT152754 NZ_CP050372:66216-66281 [Klebsiella pneumoniae]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin