Detailed information of TA system TA152708

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 90001..90254
• Replicon: plasmid unnamed3
• Accession: NZ_CP050357
• Organism: Klebsiella pneumoniae strain ZRKP01

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HB786_RS29190
• Protein ID: WP_001312851.1
• Coordinates: 90105..90254 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	90001..90060 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB786_RS29155	85779..86483	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
HB786_RS29160	86547..86708	+	162	Protein_116	DNA helicase	-
HB786_RS29165	86728..87474	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
HB786_RS29170	87529..88089	+	561	WP_000139328.1	fertility inhibition protein FinO	-
HB786_RS29175	88221..88421	+	201	WP_015059022.1	hypothetical protein	-
HB786_RS29180	88807..89406	+	600	WP_032083981.1	hypothetical protein	-
HB786_RS29185	89570..89800	+	231	WP_001736714.1	hypothetical protein	-
-	90001..90060	-	60	NuclAT_0	-	Antitoxin
-	90001..90060	-	60	NuclAT_0	-	Antitoxin
-	90001..90060	-	60	NuclAT_0	-	Antitoxin
-	90001..90060	-	60	NuclAT_0	-	Antitoxin
HB786_RS29190	90105..90254	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
HB786_RS29195	90538..90786	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-65 / blaKPC-2 / catA2	-	1..91097	91097

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T152708 WP_001312851.1 NZ_CP050357:90105-90254 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T152708 NZ_CP066430:c16544-16422 [Enterococcus faecium]
ATGAAAGGAGTAAGCCTTTTGTCCGCATATGAGACAATTCAAACCATTCTTGGTTTTGGTATGTTTACCATTGCTTTGAT
TAGTTTGATTGTAAATATGCTTAAAAATGACAAAAAGAAATAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT152708 NZ_CP050357:c90060-90001 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin