Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 49658..50385 Replicon plasmid unnamed1
Accession NZ_CP050355
Organism Klebsiella pneumoniae strain ZRKP01

Toxin (Protein)


Gene name higB Uniprot ID A0A5Q9LNL2
Locus tag HB786_RS26935 Protein ID WP_004118600.1
Coordinates 49658..49969 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag HB786_RS26940 Protein ID WP_000990392.1
Coordinates 49966..50385 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB786_RS26915 46172..46891 + 720 WP_001102107.1 (Fe-S)-binding protein -
HB786_RS26920 46902..48329 + 1428 WP_000023895.1 iron-sulfur cluster-binding protein -
HB786_RS26925 48322..49017 + 696 WP_004118595.1 lactate utilization protein C -
HB786_RS26930 49067..49447 - 381 Protein_44 gluconate permease -
HB786_RS26935 49658..49969 + 312 WP_004118600.1 type II toxin-antitoxin system HigB family toxin Toxin
HB786_RS26940 49966..50385 + 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
HB786_RS26945 50422..51624 - 1203 WP_003031541.1 hypothetical protein -
HB786_RS26950 51617..51946 - 330 WP_004118613.1 hypothetical protein -
HB786_RS26955 51943..52602 - 660 WP_000078540.1 hypothetical protein -
HB786_RS26960 53026..53619 - 594 WP_044348674.1 hypothetical protein -
HB786_RS26965 53935..54288 + 354 WP_001114073.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
HB786_RS26970 54336..54698 + 363 WP_020324593.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-65 / aac(3)-IId / blaLAP-2 / qnrS1 / aph(3')-Ia / mph(A) / sul1 / qacE / dfrA1 / tet(A) - 1..239469 239469


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12386.14 Da        Isoelectric Point: 10.0935

>T152706 WP_004118600.1 NZ_CP050355:49658-49969 [Klebsiella pneumoniae]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T152706 NZ_CP066427:515877-516218 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT152706 WP_000990392.1 NZ_CP050355:49966-50385 [Klebsiella pneumoniae]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT152706 NZ_CP066427:515647-515877 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNL2

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References