Detailed information of TA system TA152682

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 77169..77422
• Replicon: plasmid unnamed3
• Accession: NZ_CP050351
• Organism: Klebsiella pneumoniae strain ZRKP02

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HB787_RS29035
• Protein ID: WP_001312851.1
• Coordinates: 77169..77318 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	77363..77422 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB787_RS29000	72528..72943	-	416	Protein_98	IS1 family transposase	-
HB787_RS29005	73192..73593	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
HB787_RS29010	73526..73783	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
HB787_RS29015	73876..74529	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
HB787_RS29020	75468..76325	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
HB787_RS29025	76318..76392	-	75	WP_001375168.1	RepA leader peptide Tap	-
HB787_RS29030	76637..76885	-	249	WP_000083837.1	replication regulatory protein RepA	-
HB787_RS29035	77169..77318	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	77363..77422	+	60	NuclAT_0	-	Antitoxin
-	77363..77422	+	60	NuclAT_0	-	Antitoxin
-	77363..77422	+	60	NuclAT_0	-	Antitoxin
-	77363..77422	+	60	NuclAT_0	-	Antitoxin
HB787_RS29040	77623..77853	-	231	WP_001736714.1	hypothetical protein	-
HB787_RS29045	78017..78616	-	600	WP_032083981.1	hypothetical protein	-
HB787_RS29050	79002..79202	-	201	WP_015059022.1	hypothetical protein	-
HB787_RS29055	79334..79894	-	561	WP_000139328.1	fertility inhibition protein FinO	-
HB787_RS29060	79949..80695	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
HB787_RS29065	80715..80876	-	162	Protein_111	DNA helicase	-
HB787_RS29070	80940..81644	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaKPC-33 / blaCTX-M-65 / catA2	-	1..91502	91502

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T152682 WP_001312851.1 NZ_CP050351:c77318-77169 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T152682 NZ_CP066382:49698-50048 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT152682 NZ_CP050351:77363-77422 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin