Detailed information of TA system TA152680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 91902..92629
• Replicon: plasmid unnamed1
• Accession: NZ_CP050349
• Organism: Klebsiella pneumoniae strain ZRKP02

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5Q9LNL2
• Locus tag: HB787_RS27215
• Protein ID: WP_004118600.1
• Coordinates: 91902..92213 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V0AHC4
• Locus tag: HB787_RS27220
• Protein ID: WP_000990392.1
• Coordinates: 92210..92629 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB787_RS27195	88416..89135	+	720	WP_001102107.1	(Fe-S)-binding protein	-
HB787_RS27200	89146..90573	+	1428	WP_000023895.1	iron-sulfur cluster-binding protein	-
HB787_RS27205	90566..91261	+	696	WP_004118595.1	lactate utilization protein C	-
HB787_RS27210	91311..91691	-	381	Protein_97	gluconate permease	-
HB787_RS27215	91902..92213	+	312	WP_004118600.1	type II toxin-antitoxin system HigB family toxin	Toxin
HB787_RS27220	92210..92629	+	420	WP_000990392.1	helix-turn-helix domain-containing protein	Antitoxin
HB787_RS27225	92666..93868	-	1203	WP_003031541.1	hypothetical protein	-
HB787_RS27230	93861..94190	-	330	WP_004118613.1	hypothetical protein	-
HB787_RS27235	94187..94846	-	660	WP_000078540.1	hypothetical protein	-
HB787_RS27240	95270..95863	-	594	WP_044348674.1	hypothetical protein	-
HB787_RS27245	96179..96532	+	354	WP_001114073.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
HB787_RS27250	96580..96942	+	363	WP_020324593.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / sul1 / qacE / dfrA1 / tet(A) / blaLAP-2 / qnrS1 / aac(3)-IId	-	1..220761	220761

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12386.14 Da
• Isoelectric Point: 10.0935

>T152680 WP_004118600.1 NZ_CP050349:91902-92213 [Klebsiella pneumoniae]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Nucleotide

• Length: 312 bp

>T152680 NZ_CP050349:91902-92213 [Klebsiella pneumoniae]
ATGCACATTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15534.71 Da
• Isoelectric Point: 4.4702

>AT152680 WP_000990392.1 NZ_CP050349:92210-92629 [Klebsiella pneumoniae]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Nucleotide

• Length: 420 bp

>AT152680 NZ_CP050349:92210-92629 [Klebsiella pneumoniae]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Structures

Toxin

• AlphaFold DB: A0A5Q9LNL2

Antitoxin

• AlphaFold DB: V0AHC4