Detailed information of TA system TA152636

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 85157..85410
• Replicon: plasmid unnamed3
• Accession: NZ_CP050339
• Organism: Klebsiella pneumoniae strain ZRKP04

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HB789_RS29170
• Protein ID: WP_001312851.1
• Coordinates: 85261..85410 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	85157..85216 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB789_RS29135	80935..81639	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
HB789_RS29140	81703..81864	+	162	Protein_110	DNA helicase	-
HB789_RS29145	81884..82630	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
HB789_RS29150	82685..83245	+	561	WP_000139328.1	fertility inhibition protein FinO	-
HB789_RS29155	83377..83577	+	201	WP_015059022.1	hypothetical protein	-
HB789_RS29160	83963..84562	+	600	WP_032083981.1	hypothetical protein	-
HB789_RS29165	84726..84956	+	231	WP_001736714.1	hypothetical protein	-
-	85157..85216	-	60	NuclAT_0	-	Antitoxin
-	85157..85216	-	60	NuclAT_0	-	Antitoxin
-	85157..85216	-	60	NuclAT_0	-	Antitoxin
-	85157..85216	-	60	NuclAT_0	-	Antitoxin
HB789_RS29170	85261..85410	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
HB789_RS29175	85694..85942	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaKPC-2 / catA2	-	1..86253	86253

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T152636 WP_001312851.1 NZ_CP050339:85261-85410 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T152636 NZ_CP066366:959302-959637 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTCG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Nucleotide

• Length: 60 bp

>AT152636 NZ_CP050339:c85216-85157 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin