Detailed information of TA system TA152634

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 83676..84403
• Replicon: plasmid unnamed1
• Accession: NZ_CP050337
• Organism: Klebsiella pneumoniae strain ZRKP04

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5Q9LNL2
• Locus tag: HB789_RS27140
• Protein ID: WP_004118600.1
• Coordinates: 84092..84403 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V0AHC4
• Locus tag: HB789_RS27135
• Protein ID: WP_000990392.1
• Coordinates: 83676..84095 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB789_RS27105	79363..79725	-	363	WP_020324593.1	arsenite efflux transporter metallochaperone ArsD	-
HB789_RS27110	79773..80126	-	354	WP_001114073.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
HB789_RS27115	80442..81035	+	594	WP_044348674.1	hypothetical protein	-
HB789_RS27120	81459..82118	+	660	WP_000078540.1	hypothetical protein	-
HB789_RS27125	82115..82444	+	330	WP_004118613.1	hypothetical protein	-
HB789_RS27130	82437..83639	+	1203	WP_003031541.1	hypothetical protein	-
HB789_RS27135	83676..84095	-	420	WP_000990392.1	helix-turn-helix domain-containing protein	Antitoxin
HB789_RS27140	84092..84403	-	312	WP_004118600.1	type II toxin-antitoxin system HigB family toxin	Toxin
HB789_RS27145	84614..84994	+	381	Protein_85	gluconate permease	-
HB789_RS27150	85044..85739	-	696	WP_004118595.1	lactate utilization protein C	-
HB789_RS27155	85732..87159	-	1428	WP_000023895.1	iron-sulfur cluster-binding protein	-
HB789_RS27160	87170..87889	-	720	WP_001102107.1	(Fe-S)-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14 / aac(3)-IId / blaLAP-2 / qnrS1 / mph(A) / sul1 / qacE / dfrA1 / tet(A)	-	1..238333	238333

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12386.14 Da
• Isoelectric Point: 10.0935

>T152634 WP_004118600.1 NZ_CP050337:c84403-84092 [Klebsiella pneumoniae]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Nucleotide

• Length: 312 bp

>T152634 NZ_CP066366:713618-713914 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15534.71 Da
• Isoelectric Point: 4.4702

>AT152634 WP_000990392.1 NZ_CP050337:c84095-83676 [Klebsiella pneumoniae]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Nucleotide

• Length: 420 bp

>AT152634 NZ_CP066366:713916-714311 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: A0A5Q9LNL2

Antitoxin

• AlphaFold DB: V0AHC4